【摘要】 【目的】建立牡丹品种鉴定的简单重复序列(SSR)标记标准技术体系，筛选出多态性高、重复性好的牡丹SSR核心引物和参照品种，并确定品种差异判定标准，为牡丹品种的精准快速鉴定及新品种权审查提供技术支持。【方法】以9个野生牡丹种和5个具有代表性品种群、9种花型及11种花色的48个常见栽培品种为试验材料，通过收集引物信息、核对DNA序列信息、重新设计引物、分析位点多态性，筛选出SSR核心引物和参照品种，并对SSR位点组合的品种鉴别力进行分析与评价。【结果】在10个SSR位点中，9个野生种和48个品种共检测到84个等位基因。等位基因数为4～12个；多态性信息含量为0.572～0.837,均值为0.712。SSR位点组合的品种鉴别能力分析结果显示：当差异位点为1个时，至少需要5对引物才可完全区分48个品种；而当差异位点为2个时，至少需要8对引物才能完全区分48个品种。【结论】筛选出的17个参照品种和10个SSR位点在所有牡丹品种群中均表现出良好的鉴别能力。最终确定了以1个差异位点为鉴定标准的牡丹品种SSR标记技术体系。更多还原

【Abstract】 【Objective】For rapid and precise identification of peony varieties and assessment of new variety rights, identification system and benchmark were established based on SSR marker through screening reference varieties and SSR core primers with high polymorphism and good repeatability. 【Method】With 9 wild species as well as 48 common cultivated varieties, belonging to 5 groups with diverse morphology and covering 9 flower types and 11 flower colors, as materials, core SSR primers and reference varieties were screened through primer information collection, DNA sequence data verification, primer redesign, and locus polymorphism analysis. And the variety discrimination power(VDP) of SSR loci combinations were further examined and evaluated. 【Result】A total of 84 alleles were identified from the 10 analyzed SSR loci of the 9 wild species and 48 cultivated varieties. The allele number(N_a) per locus ranged from 4 to 12. The polymorphism information content(PIC) ranged from 0.572 to 0.837, with an average of 0.712. The VDP assessment of SSR loci combinations indicated that a minimum of 5 primer pairs were necessary to fully distinguish all the 48 varieties when the number of variety-specific differential locus was 1, and at least 8 primer pairs were prerequisite when the number was 2. 【Conclusion】The screened 17 reference varieties and 10 pairs of SSR core primers showed sound variety discrimination power in all the peony cultivar groups tested, and a SSR marker-based system with 1 differential locus as the benchmark was developed for the identification of peony varieties.更多还原