【摘要】 高盐低碳氮比（C/N）废水的高效脱氮处理面临着高盐胁迫与碳源不足等瓶颈，已成为制约相关产业绿色高质量发展的重大障碍。该研究将纳米零价铁（nZVI）添加至单室微生物电化学系统，探究了nZVI协同单室微生物电化学系统强化高盐低C/N含氮废水脱氮处理性能、产电效能及其机制。结果表明，nZVI与单室微生物电化学耦合系统有效提高了高盐低C/N含氮废水的脱氮处理及产电性能。当nZVI添加浓度为100 mg/L时，NH₄⁺-N与TN去除率分别提高39.9%和77.2%，脱氮过程电子消耗量增加76.8%，平均输出电压、最大功率密度和库伦效率分别提高22.2%、223.6%和8.9%。代谢活性与电子转移活性分析表明，nZVI的添加促使电极生物膜生物量、电子池容量（NAD（H））、NADH/NAD⁺、ATP含量以及电子传递系统活性（ETSA）、细胞色素c与核黄素含量显著增加（P<0.05），这可能增强了一众铁依赖型脱氮功能酶的活性及提高了脱氮功能菌的种间竞争力与抗盐胁迫能力、强化了胞外电子传递效率，从而有效促进了氮的强化去除及系统产电。该研究为高盐低C/N含氮废水的高效低碳处理提供了一定理论参考。更多还原

【Abstract】 The efficient nitrogen removal from high-salinity and low carbon/nitrogen ratio（C/N） wastewater faces bottlenecks such as high salt stress and insufficient carbon source, which has become a major obstacle restricting the green and high-quality development of related industries. In this study, nano zero-valent iron（nZVI） was added to the single-chamber microbial electrochemical systems. The enhanced nitrogen removal and electricity generation of high-salinity and low C/N wastewater via the synergistic integration of n ZVI and microbial electrochemical systems were investigated. The results showed that the nZVI and single-chamber microbial electrochemical coupling system effectively improved the nitrogen removal and electricity generation of high-salinity and low C/N nitrogen-containing wastewater. When nZVI was added at a concentration of 100 mg/L, NH₄⁺-N and TN removals were increased by 39.9% and 77.2%, respectively, and the electron consumption in the nitrogen removal process was increased by 76.8%. While the average output voltage, the maximum power density, and the coulombic efficiency were increased by 22.2%, 223.6%, and 8.9%, respectively. Metabolic activity and electron transfer activity analysis showed that the addition of nZVI significantly increased the electrode biofilm biomass, electron pool capacity（NAD（H））, NADH/NAD⁺, ATP content, and electron transport system activity（ETSA）, and cytochrome c and riboflavin contents（P<0.05）. These may enhance the activity of a series of iron-dependent nitrogen removal functional enzymes, improve the interspecies competitiveness and resistance to salt stress of nitrogen removal functional bacteria, as well as strengthen extracellular electron transfer efficiency, thus effectively promoting the enhanced removal of nitrogen and the electricity generation. This study provides some theoretical references for the efficient and low-carbon treatment of highsalinity and low C/N wastewater.更多还原