【摘要】 试验旨在研究紫玉米花青素(purple corn anthocyanin, PCA)对赤水乌骨鸡盲肠短链脂肪酸（short-chain fatty acids,SCFAs）、菌群多样性、微生物组成和丰富度的影响。采用单因素完全随机试验设计，选取144只体况良好、体重相近的8周龄赤水乌骨鸡，随机分为2组，对照组饲喂基础饲粮，试验组在基础饲粮中添加80 mg/kg的PCA，每组6个重复，每个重复12只鸡。试验持续75 d，其中预试期15 d，正试期60 d。结果表明：(1)添加80 mg/kg PCA显著提高了盲肠中丙酸、异丁酸、丁酸、异戊酸、戊酸和总短链脂肪酸含量（P<0.05）。(2)在属水平上，拟杆菌属为优势菌属，添加80 mg/kg PCA显著提高了拟杆菌属、副拟杆菌属和乳杆菌属等有益菌群的相对丰度（P<0.05）。(3)在门水平上，WPS-2菌门相对丰度与异戊酸、丙酸、异丁酸含量存在显著负相关（P<0.05），弯曲杆菌门（Campilobacterota）相对丰度与异己酸含量存在显著正相关（P<0.05）；在属水平上，unclassified＿o＿Bacteroidales菌属相对丰度与异己酸含量存在显著负相关（P<0.05）。由此可见，饲粮中添加PCA可显著改善盲肠内容物中短链脂肪酸含量与组成，显著提高拟杆菌属、副拟杆菌属和乳杆菌属菌群的相对丰度。更多还原

【Abstract】 The experiment aimed to investigate the effects of purple corn anthocyanin(PCA) on shortchain fatty acids(SCFAs), microbiota diversity, microbial composition, and richness in cecum in Chishui black-Bone chickens. A single-factor completely randomized trial design was adopted. A total of 144 healthy, similarly weighted 8-weekold Chishui Black-Bone Chickens were randomly divided into two groups: the control group was fed a basal diet, while the experimental group was fed a basal diet supplemented with 80 mg/kg of purple corn anthocyanin. Each group had six replicates, with 12 chickens per replicate. The experiment lasted for 75 days, with a pre-feeding period of 15 days and a trial period of 60 days. The results showed that:(1) adding 80 mg/kg PCA significantly increased the levels of propionic acid, isobutyric acid, butyric acid, isovaleric acid, valeric acid, and total short-chain fatty acids in the cecum(P<0.05).(2) At the genus level, Bacteroides was the dominant genus, and the addition of 80 mg/kg PCA significantly increased the relative abundance of beneficial bacterial groups such as Bacteroides, Parabacteroides, and Lactobacillus(P<0.05).(3) At the phylum level, the relative abundance of the WPS-2 phylum was significantly negatively correlated with the levels of isovaleric acid, propionic acid, and isobutyric acid(P<0.05); the relative abundance of the Campilobacterota phylum was significantly positively correlated with the level of isocaproic acid(P<0.05). At the genus level, the relative abundance of the unclassified＿o＿Bacteroidales genus was significantly negatively correlated with the level of isocaproic acid(P<0.05). These findings indicate that supplementing the diet with purple corn anthocyanins significantly improved the content and composition of short-chain fatty acids in cecum contents and significantly increased the relative abundance of Bacteroides, Parabacteroides, and Lactobacillus.更多还原