【摘要】 为了探究鸡蛔虫的遗传变异情况，对分离自安徽省凤阳县的2株鸡蛔虫线粒体ATP合成酶F0亚单位6(ATP6)基因、烟酰胺腺嘌呤二核苷酸脱氢酶亚单位Ⅰ（NAD1）基因和烟酰胺腺嘌呤二核苷酸脱氢酶亚单位Ⅱ（NAD2）基因序列进行PCR扩增和测序，用DNAStar和Dnasp6.0等软件分析3个基因的核酸序列，并用MEGA11.0构建系统进化树。结果显示，鸡蛔虫凤阳分离株ATP6、NAD1和NAD2基因的开放阅读框片段大小分别为597bp、876bp和843bp,2株鸡蛔虫3个基因的核苷酸同源性分别为99.0%、98.1%和97.8%。遗传距离分析显示，鸡蛔虫凤阳分离株的ATP6、NAD1和NAD2基因与其他鸡蛔虫同源基因的遗传距离均小于或者等于0.024,3个基因分别检测到13、7和4个单倍型，总的单倍型多样性分别为0.971、0.819和0.900，核苷酸多样性分别为0.011 41、0.006 65和0.0109 6。以ATP6、NAD1和NAD23个基因序列构建的种系进化树均显示，鸡蛔虫凤阳分离株与其他已知鸡蛔虫分离株聚类在一个分支，其次与鸽蛔虫聚类成禽蛔科支系，与其他蛔虫所属的分支均相距较远，能够鉴别不同种的蛔虫。研究表明，鸡蛔虫凤阳分离株存在遗传变异，但程度较低，且NAD2基因比ATP6、NAD1基因更适合作为鸡蛔虫遗传变异研究的分子标记。更多还原

【Abstract】 To explore the genetic variation of Ascaridia galli,the mitochondrial ATP synthase F0 subunit 6(ATP6),nicotinamide adenine dinucleotide dehydrogenase subunit 1(NAD1)and nicotinamide adenine dinucleotide dehydrogenase subunit 2(NAD2)gene sequences of two adult A.galli isolated from Fengyang of Anhui province were amplified by PCR and sequenced.The nucleic acid sequences of the three genes were analyzed by softwares such as DNAStar and Dnasp6.0,and the phylogenetic trees were constructed by MEGA11.0.The results showed that the open reading frame fragments of ATP6,NAD1and NAD2 genes of two adult A.galli Fengyang isolates were 597bp,876bp and 843bp,respectively.The nucleotide homologies of the three genes of A.galli were 99.0%,98.1% and 97.8%,respectively.Genetic distance analysis showed that the genetic distance between the ATP6,NAD1and NAD2genes of A.galli isolated from Fengyang and other homologous genes of A.galli was less than or equal to 0.024.13,7 and 4 haplotypes were determined based on ATP6,NAD1and NAD2,with total haplotype diversities being 0.971,0.819 and 0.900,the nucleotide diversities being 0.01141,0.00665 and 0.01096,respectively.Phylogenetic trees based on ATP6,NAD1and NAD2 genes showed that all A.galli isolates were clustered in one clade,and they were more closely related to Ascaridia columbae than to any other members of Ascaridata.ATP6,NAD1and NAD2 genes could identify different roundworms.The results suggested that there is genetic variation in the A.galli Fengyang isolates,but the degree is relatively low,and the NAD2 gene is more suitable as a molecular marker for studying genetic variation in A.galli than the ATP6 and NAD1 genes.更多还原