【摘要】 辽宁众辉生物科技有限公司因出水中含有大量生化难降解有机氮导致总氮超标，采用催化臭氧氧化-反硝化组合工艺进行深度处理.该工艺首先通过催化臭氧氧化将含氮有机物断链降解为无机小分子含氮化合物，再通过反硝化作用将其转化为氮气去除.以催化臭氧氧化出水作为反硝化生物处理进水，对比分析了自养、异养和混养反硝化方式的脱氮效果，以确定最优工艺.水质检测和高通量测序结果表明，异养反硝化体系对农药生化尾水的适用性及脱氮效果均优于自养和混养反硝化体系.在HRT=6 h、进水pH为7.5～8.0时，异养反硝化体系可将硝态氮浓度从56.5 mg/L降至8.2 mg/L,去除率达85%以上；总氮浓度从118.9 mg/L降至72.9 mg/L,去除率约为39%.微生物群落分析显示，异养反硝化体系的物种丰富度和多样性指数低于自养和混养反硝化体系.更多还原

【Abstract】 Liaoning Zhonghui Biotechnology Co., Ltd. uses a combined process of catalytic ozonation-denitrification for advanced treatment because its effluent contains a large amount of biochemical refractory organic nitrogen leading to excessive total nitrogen. This process first uses catalytic ozonation to break chain and degrade nitrogen-containing organic matter into inorganic small molecule nitrogen-containing compounds, then uses denitrification to convert them into nitrogen gas for removal. Using the catalytic ozonation effluent as the influent for denitrification biological treatment, it compares and analyzes the nitrogen removal effects of autotrophic, heterotrophic and mixotrophic denitrification methods to determine the optimal process. Water quality testing and high-throughput sequencing results show that the applicability and nitrogen removal effect of the heterotrophic denitrification system for pesticide biochemical tail water are better than those of the autotrophic and mixotrophic denitrification systems. When HRT=6 h and the influent pH is 7.5-8.0, the heterotrophic denitrification system reduces the nitrate nitrogen concentration from 56.5 mg/L to 8.2 mg/L, with a removal rate reaching over 85%; the total nitrogen concentration decreases from 118.9 mg/L to 72.9 mg/L, with a removal rate of approximately 39%. Microbial community analysis shows that the species richness and diversity index of the heterotrophic denitrification system are lower than those of the autotrophic and mixotrophic denitrification systems.更多还原