【摘要】 为探究野菊CiWRKY15转录因子在镉胁迫下的功能，丰富野菊遗传资源。以野菊(Chrysanthemum indicum)为材料，克隆了野菊CiWRKY15基因并转化拟南芥，测定和分析了其在镉胁迫下的各项生理指标。结果表明：野菊CiWRKY15基因开放阅读框长801 bp,编码266个氨基酸，属于WRKYⅡ亚族，受镉诱导其表达量在叶、茎、根中均显著上调。在镉胁迫条件下，过表达拟南芥株系幼苗根长更长；植株表现出更强的镉转运能力，同时叶绿素、可溶性糖、脯氨酸质量分数及抗氧化酶活性相比野生型显著增加，而过氧化氢、丙二醛质量摩尔浓度及相对电导率显著降低。说明野菊CiWRKY15基因受镉胁迫诱导，在增强镉耐性方面发挥积极作用。更多还原

【Abstract】 To investigate the function of the CiWRKY15 transcription factor from Chrysanthemum indicum under cadmium stress and to enrich the genetic resources of C. indicum, we cloned the CiWRKY15 gene from C. indicum and transformed it into Arabidopsis thaliana. We measured and analyzed various physiological parameters under cadmium stress. The results showed that the open reading frame of the CiWRKY15 gene is 801 bp long, encoding 266 amino acids, and belongs to the WRKY Ⅱ subfamily. Its expression was significantly upregulated in leaves, stems, and roots upon cadmium induction. Under cadmium stress conditions, A. thaliana seedlings overexpressing CiWRKY15 exhibited longer root lengths. The plants demonstrated enhanced cadmium transport capabilities, while the chlorophyll content, soluble sugar, proline mass fraction, and antioxidant enzyme activities significantly increased compared to the wild type. Conversely, the concentrations of hydrogen peroxide, malondialdehyde, and relative electrolyte conductivity significantly decreased. This indicates that the CiWRKY15 gene from C. indicum is induced by cadmium stress and plays a positive role in enhancing cadmium tolerance.更多还原