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2% 28-表芸·苄嘌呤可溶液剂对茶树生长发育及鲜叶生化成分含量的影响

Effects of 2% 28-homobrassinolide·benzylaminopurine Application on Growth and Biochemistry of Tea Plants

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【作者】 吴玮; 林硕; 游泳;

【Author】 WU Wei;LIN Shuo;YOU Yong;Institute of Plant Protection, Fujian Academy of Agricultural Sciences;

【通讯作者】 游泳;

【机构】 福建省农业科学院植物保护研究所;

【摘要】 【目的】研究2%28-表芸·苄嘌呤可溶液剂对茶树生长发育的调节效果,明确其对茶芽密度、鲜叶产量及生化成分的影响。【方法】采用田间随机区组设计,以清水作空白对照,设置2%28-表芸·苄嘌呤可溶液剂4个浓度梯度(6、7.5、10、15 g a.i.·hm-2)和3个对照药剂(0.004%28-表高芸苔素内酯0.02 g a.i.·hm-2、2%苄氨基嘌呤10 g a.i.·hm-2、0.136%赤·吲乙·芸苔0.102 g a.i.·hm-2)分别于3月25日和4月12日进行两次施药处理,于4月16日、4月26日和5月6日进行3次鲜叶采摘,测定茶芽密度、百芽重、鲜叶产量及茶多酚、游离氨基酸总量和咖啡碱等生化成分含量。【结果】2%28-表芸·苄嘌呤可溶液剂显著促进茶芽萌发,其中15 g a.i.·hm-2处理后3次采摘的茶芽密度较空白对照提高了4.37%~17.88%(仅第3次采摘的差异不显著),百芽重较空白对照显著提高了9.07%~17.99%(P<0.05),鲜叶平均产量增产率达13.98%。在生化成分方面,随着2%28-表芸·苄嘌呤药剂浓度的提高,茶多酚含量由13.45%提高至15.30%,咖啡碱含量由4.20%增至5.63%,酚氨比由5.51提升至7.59,且与空白对照和3个对照药剂处理比均达到显著差异(P<0.05);而游离氨基酸总量在不同药剂处理间差异不显著(P>0.05)。【结论】2%28-表芸·苄嘌呤可溶液剂能有效提高茶芽密度、百芽重和鲜叶产量,同时能提高鲜叶茶多酚与咖啡碱含量。

【Abstract】 【Objective】Effects of applying plant growth regulator, 2% 28-homobrassinolide·benzylaminopurine(28-HBR·BAP), on the growth and biochemistry of tea bushes were studied. 【Method】 A randomized complete block design using water as blank control(CK) and 3 reference agents, including 2% 28-homobrassinolide(28-HBR) aqueous solution at the rate of 0.02 g a.i.·hm-2, 2% benzylaminopurine(BAP) soluble concentrate at 10 g a.i.·hm-2, and 0.136%GA3·IAA·BR wettable powder at 0.102 g a.i.·hm-2, 28-HBR·BAP soluble concentrate at the rate of 6, 7.5, 10, and15 g a.i.·hm-2 were applied on the lots for the field experimentation. Leaves and buds were plucked from the tea bushes on April 16, April 26, and May 6 to determine the bud density, hundred-bud weight, and fresh leaf yield, as well as the contents of tea polyphenols, caffeine, and total free amino acids.【Result】 The 2% 28-HBR·BAP application significantly stimulated the bud sprouting on the plants. At the rate of 15 g a.i.·hm-2, increases over CK of 4.37%-17.88%on bud density, 9.07%-17.99% on hundred-bud weight, and 13.98% on average fresh leaf yield were observed.Biochemically, the increasing dosage significantly raised the contents of tea polyphenol from 13.45% to 15.30%, caffeine from 4.20% to 5.63%, and polyphenol-amino acid ratio from 5.51 to 7.59, except total free amino acid, in comparison to CK and 3 reference agents refered above.【Conclusion】A significant growth promoting effect with increased fresh leaf yield and elevated tea polyphenols and caffeine contents on the tea bushes were observed by the application of 2% 28-HBR·BAP.

【基金】 福建省属公益类科研院所基本科研项目——自主选题项目(2023R1022003);福建省农业科学院植物保护创新团队项目(CXTD2021027);“5511”协同创新工程(XTCXGC2021011,XTCXGC2021017);中央引导地方科技发展专项(2023L3021)
  • 【分类号】S571.1
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