【摘要】 目的 基于代谢组学联合转录组学探索电针改善多囊卵巢综合征(PCOS)脂质代谢机制。方法 将18只SD大鼠分为对照组、PCOS组和电针组，每组6只。PCOS组和电针组采用灌胃来曲唑建立PCOS模型，模型建立后电针组采用电针进行干预；对照组仅采用羧甲基纤维素钠灌胃。观察3组大鼠体重、动情周期变化；检测3组大鼠性激素、血脂、肝功能、卵巢形态、血浆代谢和肝功能指标；采用代谢组学分析血浆中代谢物的差异；采用RT-qPCR检测肝脏中基因表达的差异。结果 PCOS组体重增加、动情周期紊乱；卵巢形态显示颗粒细胞层减薄，黄体数和成熟卵泡数明显减少；性激素异常(总睾酮和雄激素水平升高，雌二醇和黄体酮水平降低)、血脂代谢紊乱(TC、TG、LDL-C升高、HDL-C水平降低)和肝功能异常(AST、总蛋白、ALB、TBIL升高)。电针组体重明显减轻，动情周期改善，且卵巢颗粒细胞层变厚，黄体和成熟卵泡数量明显增加；性激素水平改善(雄激素和总睾酮水平降低，雌二醇水平升高);血脂平衡恢复(TC、TG、LDL-C水平降低，HDL-C水平升高)和肝功能(AST、总蛋白、ALB、TBIL水平降低)。转录组学提示，电针通过下调与类固醇激素和脂质代谢相关的细胞色素P450(CYPs)家族基因表达，且RT-qPCR证实结果。联合组学分析表明电针可能主要通过亚油酸代谢途径发挥改善PCOS大鼠脂质代谢紊乱的作用。结论 电针可调节PCOS大鼠脂质代谢。更多还原

【Abstract】 Objective To explore the mechanism of electroacupuncture in improving lipid metabolism in polycystic ovary syndrome(PCOS) based on metabolomics combined with transcriptomics.Methods Eighteen SD rats were divided into the control group, the PCOS group and the electroacupuncture group, with 6 rats in each group.The PCOS group and the electroacupuncture group used intragastric letrozole to establish the PCOS model.After the model was established, the electroacupuncture group was intervened with electroacupuncture, while the control group was only given sodium carboxymethyl cellulose by gavage.Observed the changes in body weight and estrous cycle of the three groups, detected the sex hormones, blood lipids, liver function, ovarian morphology, plasma metabolism and liver function indicators of the three groups.Metabolomics was used to analyze the differences in metabolites in plasma, the differences in gene expression in the liver were detected by RT-qPCR.Results Weight gain and estrous cycle disorder occurred in the PCOS group, and the ovarian morphology showed a thinning of the granulosa cell layer, with a significant reduction in the number of corpus luteum and mature follicles.Meanwhile, sex hormones were abnormal(total testosterone and androgen levels were increased, estradiol and progesterone levels were decreased),dyslipidemia occurred(TC,TG,LDL-C were increased, HDL-C level was decreased),and liver function was abnormal(AST,total protein, ALB,TBIL were increased).The electroacupuncture group significantly reduced weight.It improved the estrous cycle, and the granulosa cell layer of the ovary thickened, with a significant increasing in the number of corpus luteum and mature follicles.Meanwhile, sex hormone levels were improved(androgen and total testosterone levels were decreased, estradiol level was increased),lipid balance(TC,TG,LDL-C levels were reduced, HDL-C level was increased) and liver function(AST,total protein, ALB,and TBIL levels were decreased) was restored.Transcriptomics suggested that electroacupuncture down-regulated the expression of cytochrome P450(CYPs) family genes related to steroid hormones and lipid metabolism, and the results were confirmed by RT-qPCR.Combined omics analysis revealed that electroacupuncture might mainly exert its effect on improving lipid metabolism disorders in PCOS rats through the linoleic acid metabolic pathway.Conclusion Electroacupuncture can regulate lipid metabolism in rats with PCOS.更多还原