【摘要】 为探究引起父母代种鸡腿部瘫痪、关节肿胀的病原并制定有效的治疗方案，本研究收集山东省某肉种鸡场表现瘸腿、瘫痪症状的病鸡，采用改良Frey氏培养基成功分离与纯化了滑液囊支原体（Mycoplasma Synoviae, MS）。随后，利用PCR技术扩增了MS 16S rRNA基因序列，并进行序列比对。同时采用MS野毒株PCR检测试剂盒对分离菌株进行鉴定，并测定不同抗生素对分离菌株的最小抑菌浓度（MIC）和药物的临床应用评估。结果显示，从山东省某肉种鸡场具有瘸腿、瘫痪症状的患病鸡中成功分离并鉴定出一株MS，命名为MS-17103株。该菌株具有典型的支原体菌落形态，经PCR鉴定为MS野毒株阳性。药敏试验结果显示，MS-17103株对盐酸沃尼妙林（MIC为0.00076mg/L）和泰万菌素（MIC为0.00153mg/L）敏感。在感染过MS的种鸡场进行药物应用，结果显示两种药物均能有效降低鸡群感染MS的阳性率。本研究结果为后期该病的治疗方案提供了重要的参考依据。更多还原

【Abstract】 To investigate the pathogen causing leg paralysis and joint swelling in parental breeder chickens and develop effective treatment strategies, sick chickens exhibiting lameness and paralysis symptoms were collected from a broiler breeder farm in Shandong Province. The Mycoplasma synoviae(MS) was successfully isolated and purified using modified Frey’s medium.Subsequently, the 16S rRNA gene sequence of MS was amplified using PCR technology and subjected to sequence alignment. Additionally, the isolated strain was identified using an MS wild-type strain PCR detection kit. Finally, the minimum inhibitory concentrations(MICs) of various antibiotics against the isolated strain were determined, and a clinical evaluation of the drugs was conducted. The results showed that a strain of MS, named MS-17103, was successfully isolated and identified from sick chickens with lameness and paralysis symptoms collected from a meat breeder chicken farm in Shandong Province. This strain exhibited typical mycoplasma colony morphology and was confirmed as MS wild-type strain-positive by PCR. The drug sensitivity test revealed that MS-17103 was sensitive to valnemulin hydrochloride(MIC of 0.00076 mg/L) and tylosin tartrate(MIC of 0.00153 mg/L). The application of these two drugs in breeder chicken farms previously infected with MS showed a significant reduction in the positive rate of MS infection in the chicken population. This study provides an important reference for the subsequent development of treatment plans for this disease.更多还原