【摘要】 随着肥胖问题的日益突出，调节血脂、预防肥胖成了人们的关注焦点，通过健康饮食方法解决该问题是最简单可行的途径。试验采用白叶1号发酵茶配伍枸杞、山楂制成养生茶，就其对小鼠3T3-L1前脂肪细胞的调脂效果和作用机理进行了研究。将3T3-L1细胞诱导为成熟脂肪细胞后，通过测定油红O染色面积、脂肪和甘油三酯（TG）含量来判断养生茶对脂质形成的抑制作用。结果显示，在200～500μg/mL范围内，随着养生茶质量浓度的升高，3T3-L1前脂肪细胞的油红O染色面积下降，表明养生茶具有降脂功效。当茶样质量浓度为300、400、500μg/mL时，与模型组相比，细胞脂肪含量分别降低了22.64、23.17、30.63个百分点，降脂效果优于阳性药物辛伐他丁。此外，养生茶可以显著减少细胞中的TG含量，具有剂量依赖效应。当养生茶质量浓度为500μg/mL时，细胞内TG含量最低，与模型组相比降低了89.33%。通过茶饮代谢物与3T3-L1细胞脂肪指标的相关性分析发现，该养生茶中多种成分与3T3-L1细胞的脂质代谢物呈强相关关系。本研究可为开发具有降脂功能的新式茶饮产品提供科学依据。更多还原

【Abstract】 With the increasing problem of obesity, lipid-regulation and weight prevention have become the pursuit goals for many people. It is the easiest and feasible way to solve this problem by healthy diet. The lipid-regulation effect and potential mechanism of health tea drink formulated with fermented albino tea(Camellia sinensis), Lycium barbarum,and Crataegi fructus were investigated in 3T3-L1 preadipocytes. Inducing 3T3-L1 cells into mature adipocytes, the inhibitory effects of the health tea drink were determined by oil red O staining area, fat and triglyceride(TG) contents.The results show that the oil red O staining area of 3T3-L1 preadipocytes decreased with the increase of the concentration of health tea drink in the range of 200-500 μg/m L, indicating that health tea drink had lipid-lowering effect. When the tea drink concentration was 300, 400 and 500 μg/m L, compared with the model group, the fat content was reduced by22.64%, 23.17%, 30.63%, respectively, which were better than the positive drug group(simvastatin). Additionally, health tea drink could significantly reduce the TG content in cells with a dose-dependent effect. When the health tea drink concentration was 500 μg/m L, the TG content was the lowest, which was 89.33% lower than that of the model group.Through the correlation analysis between metabolites in tea drink and fat indicators, it was found that various compounds in tea drink were significantly correlated with the fat metabolism of 3T3-L1 cells. This study provided a scientific basis for the development of new tea products with lipid-regulation function.更多还原