【摘要】 目的：研究补肾强督方对人骨髓间充质干细胞（hBMSCs）成骨分化的影响及其作用机制。方法：用补肾强督方含药血清处理hBMSCs，诱导成骨分化14 d，茜素红染色评估矿化程度，RT-PCR检测成骨细胞标志物及H19、miR-22的表达，Western Blot检测β-catenin和DKK-1的表达。构建H19过表达的hBMSCs，并加入补肾强督方高剂量含药血清干预，诱导成骨分化14 d后再次检测上述指标。结果：与空白对照组比较，补肾强督方含药血清抑制hBMSCs成骨分化，补肾强督方高剂量组可降低Wnt通路中的β-catenin表达（P<0.01），上调DKK-1的表达（P<0.01），并降低H19的表达（P<0.05），增加miR-22的表达（P<0.01）。与H19过表达组比较，补肾强督方高剂量含药血清能部分挽救H19过表达引起的hBMSCs成骨分化（P<0.01,P<0.05），调节DKK-1和β-catenin的表达（P<0.01,P<0.05）。结论：补肾强督方可能通过lncRNA H19/miR-22/Wnt通路干预hBMSCs的成骨分化，为该方在AS病理性成骨中的应用提供了理论依据。更多还原

【Abstract】 Objective: To investigate the effects and mechanisms of Bushen Qiangdu Formula(BSQD) on the osteogenic differentiation of human bone marrow mesenchymal stem cells(hBMSCs). Methods: hBMSCs were treated with BSQDcontaining serum to induce osteogenic differentiation for 14 d. Alizarin Red staining was performed to evaluate the degree of mineralization. RT-PCR was used to detect osteogenic cell markers, as well as the expression of H19 and miR-22. Western Blot was conducted to examine the expression of β-catenin and DKK-1. hBMSCs with overexpression of H19 were constructed,and high-dose BSQD-containing serum was added to intervene. After inducing osteogenic differentiation for 14 d, the abovementioned indicators were re-evaluated. Results: Compared with control group, BSQD-containing serum could inhibit the osteogenic differentiation of hBMSC, high-dose BSQD-containing serum could reduce the expression of β-catenin in the Wnt pathway(P<0.01), upregulate the expression of DDK-1(P<0.01), and decrease the expression of H19(P<0.05) while increasing the expression of miR-22(P<0.01). Compared with H19 overexpression group, high-dose BSQD-containing serum could partially rescue the osteogenesis caused by H19 overexpression in hBMSCs(P<0.01, P<0.05), regulate the expression of DKK-1 and β-catenin(P<0.01, P<0.05). Conclusion: BSQD may intervene in the bone formation and differentiation of hBMSCs through the lncRNA H19/miR-22/Wnt pathway, providing a theoretical basis for the application of BSQD in pathological osteogenesis.更多还原