【摘要】 目的:运用网络药理学方法和分子对接技术研究沙棘黄酮抗动脉粥样硬化(atherosclerosis, AS)的核心成分和作用机制。方法:通过TCMSP数据库查找沙棘有效成分与靶点，通过Genecards和OMIM数据库搜集AS靶点，利用Venny 2.1获得交集靶点，采用Cytoscape 3.7.2绘制PPI网络，运用Metascape平台对筛选到的靶点进行GO功能分析和KEGG通路富集分析；最后应用AutoDock和PyMOL软件进行分子对接。结果:筛选出11个活性成分，224个靶点，与AS交集靶点80个，GO功能分析得到共有条目5 242个，KEGG通路分析富集在234个通路上，其中流体剪切应力与动脉粥样硬化通路、PI3K-Akt信号通路和VEGF信号通路等为关键通路。分子对接结果显示沙棘主要活性成分槲皮素、异鼠李素和豆甾醇与靶点IL1β、PPARG、ADRB2和NOS3对接较好。结论:沙棘中黄酮类物质槲皮素和异鼠李素可能通过调控流体剪切应力与动脉粥样硬化通路、PI3K-Akt信号通路和VEGF信号通路等，作用于IL1β、PPARG、ADRB2、NR3C1和NOS3等相关枢纽靶点，从而起到抗炎、调节氧化应激及脂代谢紊乱，修复血管损伤等而达到抗AS的功效，这为后续基础研究提供了理论依据。更多还原

【Abstract】 Objective:To study the core components and mechanism of sea buckthorn flavonoids anti-atherosclerosis(anti-AS) by network pharmacology and molecular docking technology. Methods: The active components and targets of Sea buckthorn were searched by TCMSP database, AS targets were collected by Genecards and OMIM database, intersection targets were obtained by Venny 2.1, and PPI networks were mapped by Cytoscape 3.7.2. GO function analysis and KEGG pathway enrichment analysis were performed on the selected targets using Metascape platform. Finally, AutoDock and PyMOL software were used for molecular docking. Results:11 active ingredients, 224 targets and 80 intersection targets with AS were selected. A total of 5 242 items were identified by GO functional analysis, and 234 pathways were enriched by KEGG pathway analysis, among which fluid shear stress and atherosclerosis pathway, PI3K-Akt signaling pathway and VEGF signaling pathway were key pathways. Molecular docking results showed that the main active components of sea buckthorn quercetin, isorhamnetin and stigasterol had good docking with the target IL1β, PPARG, ADRB2 and NOS3. Conclusion: Quercetin and isorhamnetin in sea buckthorn may act on IL1β, PPARG, ADRB2, NR3C1 and NOS3 targets of fluid shear stress and atherosclerosis pathway, PI3K-Akt signaling pathway and VEGF signaling pathway, thus, it can achieve anti-AS effect by anti-inflammation, regulating oxidative stress, lipid metabolism disorders, and repairing vascular damage, etc., which provides a theoretical basis for subsequent basic research.更多还原