【摘要】 为了明确植物乳杆菌脂磷壁酸对肠炎的作用，提取了益生性植物乳杆菌GSLP-7的脂磷壁酸(lipoteichoic acid, LTA),灌胃肠炎小鼠，检测小鼠肠道组织损伤、血清炎症因子、髓过氧化物酶(myeloperoxidase, MPO)和抗氧化物活性、粪便中短链脂肪酸的含量以及免疫信号通路相关蛋白表达水平的变化，结果显示，脂磷壁酸能有效缓解肠道炎症，效果优于益生菌菌体GSLP-7。与肠炎小鼠相比，脂磷壁酸干预能够显著减轻小鼠结肠组织病理学损伤，结肠萎缩减少12.38%;血清中促炎相关细胞因子TNF-α、IL-1β、IL-6、IL-12含量分别降低了34.97%、52.47%、38.59%、34.30%,抗炎相关细胞因子IL-10的含量增加了24.78%(P<0.05);结肠组织的MPO和血清中的MPO活性、一氧化氮(NO)和丙二醛(malondialdehyde, MDA)的含量分别降低了27.93%、23.84%、29.60%、35.32%,总超氧化物歧化酶(total superoxide dismutase, T-SOD)和谷胱甘肽(glutathione, GSH)活性增加了56.86%、39.74%;肠道短链脂肪酸总量提高了3.92倍，表明脂磷壁酸干预后肠道炎症缓解(P<0.05)。差异表达蛋白的Western-blot检测显示，抑制肠上皮细胞TLR4, MyD88和MAPK关键蛋白的表达，阻断TLR4-MyD88-MAPK和NF-κB信号通路，是脂磷壁酸缓解肠炎的可能机制。更多还原

【Abstract】 To clarify the effect of lipoteichoic acid produced by Lactobacillus plantarum on enteritis, lipoteichoic acid from a probiotic Lactobacillus plantarum GSLP-7 was extracted, and gavaged to mice with enteritis. The intestinal tissue damage of mice, level of serum cytokines, activity of myeloperoxidase(MPO) and antioxidants, content of short-chain fatty acids in feces, and protein expression levels related to immune signaling pathways were examined. The results showed that lipoteichoic acid could effectively relieve intestinal inflammation, and its effect was better than that of probiotic Lactobacillus plantarum GSLP-7. Compared with the group of mice with enteritis, the lipoteichoic acid intervention significantly reduced the histopathological damage of colon in mice and reduced colonic atrophy by 12.38%. Lipoteichoic acid decreased the content of cytokines TNF-α, IL-1β, IL-6, and IL-12 in serum by 34.97%, 52.47%, 38.59%, and 34.30%, respectively, and increased the content of anti-inflammatory cytokine IL-10 by 24.78%(P<0.05). It decreased the tissue MPO and serum MPO activity, and nitric oxide and malondialdehyde content by 27.93%, 23.84%, 29.60%, 35.32% respectively, and increased total superoxide dismutase and glutathione activities by 56.86% and 39.74%. It also increased the total amount of intestinal short-chain fatty acids by 3.92 times to that of the mice with enteritis. These results indicated that intestinal inflammation was relieved by lipoteichoic acid intervention(P<0.05). Results of differentially expressed proteins determined by Western-blot showed that lipoteichoic acid inhibitted the expression of key proteins TLR4, MyD88 and MAPK in intestinal epithelial cells and blocked the TLR4-MyD88-MAPK and NF-κB signal pathyway, which might be the possible mechanism of mitigated effect of lipoteichoic acid on enteritis.更多还原