【摘要】 【目的】基于网络药理学探讨桂附散(GFS)对猪繁殖与呼吸综合征病毒(PRRSV)的抑制作用。【方法】通过TCMSP、HERB和ETCM数据库筛选GFS的有效成分及作用靶点，结合CTD、OMIM、GeneCards数据库查找PRRSV的相关靶点，通过STRING数据库构建蛋白互作(PPI)网络；运用Cytoscape 3.7.2软件筛选出核心靶点，导入DAVID数据库进行基因本体(GO)功能富集分析和京都基因与基因组百科全书(KEGG)通路富集分析，并构建药物-疾病-靶点-通路网络。【结果】结果表明，GFS中发挥抗PRRSV的活性成分有槲皮素、美迪紫檀素、山柰酚、刺芒柄花素、5-甲基-7-甲氧基异黄酮等共136个，核心作用靶点有肿瘤坏死因子、核转录激活蛋白1、细胞肿瘤抗原p53、胱天蛋白酶3和丝裂原激活蛋白激酶14,GO功能和KEGG通路富集分析显示，靶点作用包含生物过程190条、细胞组分14条和分子功能36条，参与趋化因子信号通路、黏着斑、生长激素合成分泌和作用、坏死性凋亡等代谢通路。【结论】综上所述，通过筛选GFS抑制PRRSV的有效成分、关键作用靶点及代谢通路，为GFS抗PRRSV的应用提供了参考依据。更多还原

【Abstract】 [Objective]To explore the mechanism of Guifusan(GFS) in treating porcine reproductive and respiratory syndrome virus(PRRSV) based on network pharmacology in this study.[Methods]Using TCMSP,HERB,and ETCM databases to screen drug component targets, searching for PRRSV related targets through CTD,OMIM,and GeneCards databases, and uploading it to the STRING database to establish a protein interaction(PPI) network; using Cytoscape 3.7.2 software to identify its core targets, using the DAVID database to conduct GO function and KEGG pathway enrichment analysis on core targets, and construct a drug-disease-target-pathway network.[Results]The results of network pharmacology showed that there were 136 active ingredients in GFS for PRRSV,such as quercetin, medallin, kaempferol, formononetin, 5-methyl-7-methoxyisoflavone, etc, the core targets of GFS were TNF,JUN,TP53,CASP3 and MAPK14,which were mainly used for chemokine signaling pathway, adhesive spot, growth hormone synthesis, secretion and function, necrotic apoptosis, etc.[Conclusion]In summary, this study screened the active ingredients, key targets and metabolic pathways of GFS for PRRSV,which provided a reference for the application of GFS in the treatment of PRRSV.更多还原