【摘要】 目的 考察全血采集后不同保存温度和时间对凝血因子Ⅷ(FactorⅧ,FⅧ)和凝血因子Ⅸ(FactorⅨ,FⅨ)活性的影响，为选择最佳保存条件提供数据支持。方法 采集本单位20名健康志愿者全血各16 mL,分装到8个枸橼酸钠抗凝管中。立即取2管离心后进行FⅧ、FⅨ活性水平检测；其余6管全血分别置于常温和低温条件下，在2、4、6 h分别取全血离心后检测FⅧ、FⅨ活性水平。以即刻检测的2管结果均数作为对照组，其它时间段为试验组进行比较，采用SPSS 26.0进行统计分析。结果 全血在常温和低温放置4 h, FⅧ活性都有较好的稳定性(116.53±25.95 vs 125.22±27.33,109.77±23.23 vs 125.22±27.33)(P均>0.05);在6 h时，FⅧ活性下降与对照组相比有统计学差异(108.65±22.92 vs 125.22±27.33,100.46±20.19 vs 125.22±27.33)(P均<0.05),但常温保存更接近对照组检测结果。全血在常温和低温放置6 h, FⅨ活性仍有较好的稳定性(97.14±19.48 vs 96.76±19.67,97.10±17.45 vs 96.76±19.6)(P均>0.05)。结论 采集后的全血标本，在常温或低温保存，4 h内检测不影响检测结果的准确性。全血存放6 h, FⅨ的活性能保持稳定；但FⅧ活性显著降低。在6 h内全血中FⅧ活性在常温稳定性优于低温条件。更多还原

【Abstract】 Objective To investigate the effects of different storage temperatures and durations on the activities of coagulation factor Ⅷ(Factor Ⅷ, FⅧ) and coagulation factor Ⅸ(Factor Ⅸ, FⅨ) after whole blood collection, so as to provide data support for the optimal storage conditions. Methods A total of 16 mL of whole blood was collected from each of the 20 healthy volunteers at our blood center and aliquoted into 8 sodium citrate anticoagulant tubes. Two tubes were immediately centrifuged for the measurement of FⅧ and FⅨ activity levels. The remaining 6 tubes of whole blood were respectively stored under room temperature and low-temperature conditions. At 2, 4, and 6 h, the whole blood samples were centrifuged and analyzed for FⅧ and FⅨ activity levels. The mean values of the two immediately tested tubes were used as the control group, while other tubes were designated as the experimental groups for comparison. Statistical analysis was performed using SPSS 26.0. Results The activity of FⅧ in whole blood remained stable after 4 hours of storage at both room temperature and low temperature(116.53±25.95 vs 125.22±27.33,109.77±23.23 vs 125.22±27.33)(P>0.05 for both). However, by 6 hours, FⅧ activity showed a statistically significant decline compared to the control group(108.65±22.92 vs 125.22±27.33, 100.46±20.19 vs 125.22±27.33)(P<0.05 for both), though the room temperature group results were closer to the control values. The activity of FⅨ in whole blood remained stable after 6 hours of storage under both conditions(97.14±19.48 vs 96.76±19.67, 97.10±17.45 vs 96.76±19.6)(P>0.05 for all comparisons). Conclusion For whole blood samples after collection, storage at either room temperature or low temperature for up to 4 hours does not compromise the accuracy of test results. When stored for 6 hours, FⅨ activity remains stable, whereas FⅧ activity decreases significantly. Notably, FⅧ activity demonstrates better stability at room temperature than under low-temperature conditions within the 6-hour storage.更多还原