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西瓜根结线虫病生防细菌的筛选鉴定及促生效果评价
Screening, Identification and Evaluation of Growth-promoting Effect of Biocontrol Bacteria Against Watermelon Root-knot Nematode Disease
【摘要】 以施加过黑曲霉Y61菌剂和哈茨木霉菌剂的西瓜根际土壤为试验样本,采用土壤稀释分离法获得杀南方根结线虫的生防细菌,研究了不同生防细菌对南方根结线虫2龄幼虫的致死率和对植物促生指标的影响,以期筛选出具有良好应用开发前景的菌株。结果表明:粘质沙雷氏菌(Serratia marcescens)对南方根结线虫2龄幼虫的致死率达到100%,盆栽防效为77%,对西瓜种子的发芽具有明显促进作用,能够利用葡萄糖、蔗糖、木糖、甘露醇、麦芽糖、山梨醇等,显示出良好的应用开发前景。
【Abstract】 Taking the rhizosphere soil of watermelon treated with Aspergillus niger Y61 and Trichoderma harzianum as the experimental samples,the biocontrol bacteria that kill Meloidogyne incognita were obtained by soil dilution and separation method.The mortality rate of different biocontrol bacteria on the second-stage juvenile of M.incognitaand their effects on the plant growth promotion indicators were studied,with the aim of screening out strains with good application and development prospects.The results showed that the mortality rate of Serratia marcescens against the second-stage juvenile of M.incognita reached 100%,the pot control effect was 77%,and it had a significant promoting effect on the germination of watermelon seeds.It could utilize glucose,sucrose,xylose,mannitol,maltose,sorbitol,etc.,this strain showed a good prospect of application development.
【Key words】 watermelon; Meloidogyne incognita; biocontrol bacterium; Serratia marcescens;
- 【文献出处】 北方园艺 ,Northern Horticulture , 编辑部邮箱 ,2025年19期
- 【分类号】S436.5;S476
- 【下载频次】117