【摘要】 [目的]提高生防菌株抑菌活性。[方法]以前期筛选的大豆根腐病拮抗菌株解淀粉芽孢杆菌为试验材料，选取不同碳源、氮源、无机盐等培养基组分，同时设置生防菌株在不同发酵条件下，对靶标菌尖孢镰刀菌的抑制作用进行平皿测定。[结果]菌株BQD1在优化单因素培养条件下对靶标菌均有抑制作用，且当培养基配比为蔗糖2%,蛋白胨1.5%,磷酸氢二钾0.15%,氯化钠0.15%,且初始pH 6.0、装液量100 mL、接种量2%、摇床转速180 r/min、培养温度30℃、发酵72 h时，对靶标菌的抑菌活性最高。[结论]菌株BQD1生防潜力有待进一步挖掘。更多还原

【Abstract】 [Objective] In order to improve the inhibition activity of the biocontrol strains. [Method]The previously screened-out Bacillus amyloliquefaciens against soybean root rot was selected. Different carbon sources, nitrogen sources, inorganic salts and fermentation conditions were tested to investigate the inhibitory effects on the target Fusarium oxysporum by plate assay. [Result] Strain BQD1 exhibited inhibitory effects against the target Fusarium oxysporum under the optimized single-factor culture conditions. Furthermore, the inhibitory activity was maximized when the culture medium consisted of 2% sucrose, 1.5% peptone, 0.15% potassium dihydrogen phosphate, and 0.15% sodium chloride, with an initial pH of 6.0, a liquid volume of 100 mL, an inoculation volume of 2%, a shaking speed of 180 r/min, a culture temperature of 30 ℃, and a fermentation duration of 72 h. [Conclusion] The potential biocontrol control of strain BQD1 needs further exploration.更多还原