【摘要】 为了筛选能够提高羊免疫应答的Toll样受体激动剂，设计针对羊TLR1～TLR10基因序列的引物，采用PCR方法检测羊体内Toll样受体的表达情况。然后，将人载脂蛋白A1(ApoA1)和HMT13佐剂配伍不同的TLR1～TLR9样受体激动剂，分组免疫湖羊后采集血样，并通过琼脂扩散试验检测抗体效价。结果表明：在湖羊的血液中均可检测到TLR1～TLR10的特异性目的片段。经过3、5、7次免疫后，添加TLR3激动剂的组效价最高，说明TLR3激动剂Ploy(I∶C)免疫增强效果最佳，可作为提高羊疫苗免疫的候选激动剂。更多还原

【Abstract】 In order to select Toll-like agonists that can enhance the immune response in sheep, PCR method was used to design the primers for the gene sequences of TLR1-TLR10 for sheep, and detect the expression of Toll-like receptors in sheep.Subsequently, human apolipoprotein A1(ApoA1) and HMT13 adjuvant were combined with various commercialized TLR1-TLR9 agonists. Post immunization of Hu Sheep in different groups, blood samples were collected and antibody titers were detected by using agar diffusion test. The results indicated the specific target fragments of TLR1-TLR10 were detected in the blood of Hu Sheep. After 3, 5, and 7 rounds of immunization, the titer in TLR3 agonist group was the highest, which suggested that the TLR3 agonist Poly(I∶ C) exhibited the best immuno-enhancing effect, so it could be used as a candidate agonist for improving the vaccine immunogenicity in sheep.更多还原