【摘要】 目的 探索在本科生中开展流式细胞术的实验教学与实践，激发本科生的主观能动性，将学到的理论知识进行拓展应用，以便应用到未来的科研中。方法 文章以参与“橘井青苗”计划的本科生为研究对象，层次递进式开展流式细胞术实验方法课程的实践与初探。此实践计划设置了2个递进层次的教学科研一体的实践范例，初阶为小鼠成肌细胞缺氧造模，并通过CCK-8法检测细胞的存活率，进阶为应用流式细胞仪检测小鼠成肌细胞的凋亡情况等。结果 本科生在一周时间内全程参与到实践计划中，通过实验设计和实践，学生掌握细胞扩增、细胞存活率检测、细胞凋亡检测与数据分析，提高了学生的动手能力和创新能力，调查问卷课程满意度达到95%以上。结论 层次递进式开展流式细胞术实验方法课程适合本科生，这种融合科研创新思路、课题设计与执行能力的教学培养方法，能带动学生在实验研究中进行科学规划和使用大型仪器。更多还原

【Abstract】 Objective To explore the experimental teaching and practice of flow cytometry among undergraduates, which could stimulate the subjective initiative of undergraduates, and let them expand and apply the theoretical knowledge into their future scientific research. Methods In this paper, the undergraduate students who participated in the “Jujing-Qingmiao” program were selected as the research objects, and the practice and preliminary exploration of flow cytometry experimental methods were carried out step by step.There were two progressive levels of integrated teaching and scientific research practice examples, and the hypoxia modeling of mouse myoblast cells and the survival rate of cells detected by CCK-8 method were designed in the first stage, and the apoptosis of mouse myoblast cells detected by the flow cytometry was in the senior stage. Results Undergraduates were fully involved in the practice plan within one week. Through experimental design and practice, students mastered cell amplification, cell survival rate detection, cell apoptosis detection and data analysis, which improved students’ hands-on ability and innovation ability. More than 95% students were satisficed about this program according to the questionnaires. Conclusion The progressive flow cytometry course is suitable for undergraduate students in our university. This teaching and training method, which integrated innovative ideas of scientific research,project design and execution ability, can promote students to design and operate large-scale instruments in experimental research.更多还原