【摘要】 目的 探讨丹参多酚酸盐调控NF-κB信号通路对冠心病大鼠心肌细胞凋亡的影响。方法 选取60只健康雄性的SD大鼠，将其随机分为对照组、模型组、阳性对照组及丹参多酚酸盐低、中、高剂量组，每组10只。模型组、阳性对照组、丹参多酚酸盐低中高剂量组在连续6周每天喂食高脂饲料后每隔24 h腹腔注射30μg/kg垂体后叶素，连续3次，建立模型。丹参多酚酸盐低、中、高剂量组分别给予50、150和450 mg/L丹参多酚酸盐，阳性对照组给予10 mg/kg瑞舒伐他汀，对照组采用常规饮食。观察各组大鼠心肌梗死面积、心肌细胞凋亡情况、心肌组织中炎性因子表达、心肌组织中超氧化物歧化酶（SOD）、丙二醛（MDA）表达、NF-κB通路相关蛋白及mRNA表达。结果 与对照组相比，模型组梗死心肌组织/危险区（IS/AAR）、凋亡率、炎症因子水平较高（P<0.05）；与模型组相比，丹参多酚酸盐各剂量组有所改善（P<0.05）。与对照组相比，模型组SOD较低，MDA较高（P<0.05）；与模型组相比，丹参多酚酸盐各剂量组SOD较高，MDA较低（P<0.05）。与对照组相比，模型组NF-κB p65、NF-κB蛋白及mRNA表达较高（P<0.05）；与模型组相比，丹参多酚酸盐各剂量组NF-κB p65、NF-κB蛋白及mRNA表达较低（P<0.05）。结论 丹参多酚酸盐可有效抑制冠心病大鼠心肌细胞凋亡，其作用机制与NF-κB信号通路有关。更多还原

【Abstract】 Objective To explore the effect of salvianolate regulating NF-κ B signal pathway on cardiomyocyte apoptosis in rats with coronary heart disease. Methods Sixty healthy male SD rats were selected and randomly divided into control group, model group, positive control group, salvianolate low, medium and high dose groups, with 10 rats in each group. Model group, positive control group, salvianolate low, medium and high dose group were fed high-fat diet every day for 6 weeks and intraperitoneally injected 30 μg/kg pituitoxin every 24 h for 3 consecutive times to establish the model. Salvianolate low, medium and high dose groups were given 50,150 and 450 mg/L salvianolate, respectively, while positive control group was given 10 mg/kg rosuvastatin and control group was given conventional diet. Myocardial infarction size, myocardial apoptosis, inflammatory factor expression, superoxide dismutase(SOD), malondialdehyde(MDA) expression, NF-κB pathway related protein and mRNA expression in myocardial tissue were observed. Results Compared with the control group, the infarct myocardial tissue/risk area(IS/AAR), apoptosis rate and inflammatory factor levels in the model group were higher(P<0.05). Compared with model group, salvianolate dosage groups were improved(P<0.05). Compared with the control group, SOD was lower and MDA was higher in the model group(P<0.05). Compared with model group, the SOD of salvianolate groups was higher and the MDA was lower(P<0.05). Compared with control group, the expressions of NF-κB p65, NF-κB protein and mRNA in model group were higher(P<0.05). Compared with model group, the expressions of NF-κB p65, NF-κB protein and mRNA in salvia polyphenol acid dose groups were lower(P<0.05). Conclusion Salvianolate can effectively inhibit myocardial cell apoptosis in rats with coronary heart disease, and its mechanism is related to NF-κ B signal pathway.更多还原