【摘要】 目的 建立星夏止痛贴膏的质量检测方法。方法 采用薄层色谱法（TLC）对方中所含大黄和川乌、草乌进行鉴别；采用高效液相定量色谱法，Phenomenex Luna Omega Polar 18C(4.6 mm×250 mm,5μm)色谱柱；以0.1%磷酸（A）-乙腈（B）为流动相，梯度洗脱，流速1 ml/min，检测波长235 nm，柱温25℃，进样量10μl，对星夏止痛贴膏中的苯甲酰新乌头原碱、苯甲酰乌头原碱和芦荟大黄素进行含量测定。结果薄层色谱鉴别图的斑点清晰，分离度好，且阴性对照无干扰；苯甲酰新乌头原碱在1.425 2～71.262 0μg/ml、苯甲酰乌头原碱在1.329 8～66.492 4μg/ml、芦荟大黄素在1.207 1～60.356 2μg/ml浓度范围内呈良好的线性关系，精密度和重复性好，平均加样回收率分别为98.62%、91.78%及100.04%，相对标准偏差（RSD）分别为4.62%、3.77%及4.81%(n=6)。结论 该方法简便，灵敏度高，为星夏止痛贴膏的质量制定提供了实验依据。更多还原

【Abstract】 Objective To establish the method of quality control for Xingxia Zhitong plaster. Methods The TLC was adopted to qualitatively identify for Rheum palmatum L, Aconitum carmichaelii and Aconitum kusnezoffii.High performance liquid chromatography was performed on Phenomenex Luna Omega Polar 18C(4.6 mm×250 mm)column, with its mobile phase as a gradient elution of 0.1% phosphoric acid water(A)-methanol(B)and flow rate at 1.0ml/min, column temperature maintained at 25 ℃ and detection wavelength at 235 nm,the sample size were all at 10μl. HPLC was used to determine the content of Benzoylmesaconine、Benzoylaconine and Aloe emodin in Xingxia Zhitong plaster. Results TLC spots were clear and well-separated; Benzoylmesaconine、Benzoylaconine and Aloe emodin showed a good linear relationship within a range of 1.425 2～71.262 0 μg/ml、1.329 8～66.492 4 μg/ml and 1.207 1～60.356 2 μg/ml. Their precision、repeatability and stability met the requirements. Their average recovery were 98.63%、91.78%、100.04% and RSD were 4.62%、3.77% and 4.81%(n=6). Conclusion The method is rapid,simple and accurate, it provided an experimental basis for the quality control of Xingxia Zhitong plaster.更多还原