【摘要】 目的：探讨黑老虎叶提取物在化妆品原料开发中的潜在应用。方法：以黄酮含量为评价标准，构建了大孔吸附树脂对黑老虎叶提取物的纯化流程，并结合动物实验、细胞实验及化学实验，开展提取物的急性毒性及其功能特性研究。结果：AB-8大孔吸附树脂对黑老虎叶黄酮具有良好的吸附和解吸性能；上样条件为：总黄酮浓度1.0 mg/mL的料液调节pH 3，以3 BV/h流速上样5 BV；洗脱条件为：用80%乙醇以3 BV/h流速洗脱5 BV；动物实验表明，提取物无急性毒性；细胞实验显示，在2.5～50.0μg/mL浓度范围内，提取物能显著减少H₂O₂引起的人皮肤成纤维细胞死亡，有效降低细胞中ROS、MDA水平，增强SOD、CAT活性；在0.1～2.0 mg/mL浓度范围内，提取物能显著降低小鼠B₁₆F₁₀细胞中TYR活性；提取物抑制HAase的IC₅₀为4.748μg/mL。结论：利用AB-8大孔吸附树脂纯化的黑老虎叶提取物没有表现出急性毒性，同时展现了抗衰老、美白、抗过敏等功效，在作为化妆品原料应用上具有一定开发潜力。更多还原

【Abstract】 Objective:To study the potential application of extract from Kadsura coccinea leaf in the development of cosmetic raw materials.Methods:The extract of K.coccinea leaf was purified by mac‐roporous adsorption resin,and the acute toxicity of the extract was studied by animal experiments.The anti-aging,whitening,and antiallergic effects of the extract were investigated by cell biological model experiments and chemical experiments.Results:AB-8 macroporous adsorption resin had a better puri‐fication effect on the extract of K.coccinea leaf.The loading conditions were as follows:flavonoid con‐centration of 1.0 mg/mL,pH 3,flow rate 3 BV/h,and loading volume 5 BV.The elution conditions were as follows:80%ethanol,with the same flow rate and elution volume as the loading condition.The tests of acute oral toxicity and acute dermal toxicity of the extract showed it was non-toxic.Within the range of 2.5-50.0μg/mL,the extract could significantly raise the survival rates of skin fibroblasts damaged by H₂O₂ and significantly reduce the levels of ROS and MDA,as well as the activities of SOD and CAT in damaged cells.Within the range of 0.1-2.0 mg/mL,the extract significantly reduced TYR activity in mouse B₁₆F₁₀ melanoma cells.The extract had a significant inhibitory effect on HAase,with IC₅₀ of 4.748μg/mL.Conclusion:The extract of K.coccinea leaf purified by AB-8 macroporous adsorption resin had no acute toxicity,and showed anti-aging,whitening,anti-allergy and other ef‐fects,which had certain development potential as a cosmetic raw material.更多还原