【摘要】 目的：评价鼠麴草（Gnaphalium affine D. Don）的质量。方法：采用TLC法进行定性鉴别，按2020年版《中华人民共和国药典》四部通则方法测定水分、总灰分、酸不溶性灰分和浸出物含有量，通过HPLC法测定木犀草素和芹菜素的含有量。采用SPSS 20.0软件对23批鼠麴草进行聚类分析和主成分分析。结果：TLC斑点清晰，分离度好。23批样品水分、总灰分、酸不溶性灰分和浸出物含有量分别为7.83%～10.70%、5.95%～17.11%、0.83%～9.40%和8.87%～27.43%。木犀草素和芹菜素分别在0.7～374.5μg/mL(r=0.999 9)和0.4～195.5μg/mL(r=0.999 4)范围内线性关系良好，平均加样回收率分别为98.95%(RSD=1.94%)和97.18%(RSD=3.46%)。西藏地区的样品被聚为一类，其他地区样品分布离散，分类结果与地域有关。结论：该方法稳定可靠，可用于鼠麴草的质量评价。更多还原

【Abstract】 Objective:To evaluate the quality of Gnaphalium affine D. Don. Methods:The qualitative identification was performed by TLC. The contents of water, total ash, acid-insoluble ash and extract were determined according to the methods of the four general rules of the Chinese Pharmacopoeia(2020 edition). The contents of luteolin and apigenin were determined by HPLC. Cluster analysis and principal component analysis were performed on 23 batches of G. affine by SPSS 20.0. Results:The TLC spots were clear with good resolution. The content of water, total ash, acid-insoluble ash and extract were 7.83% ～ 10.70%, 5.95% ～ 17.11%, 0.83% ～ 9.40% and 8.87% ～ 27.43% in 23 batches of samples, respectively. Luteolin and apigenin showed good linear relationships within the ranges of 0.7 ～374.5 μg/mL(r = 0.999 9) and 0.4 ～ 195.5 μg/mL(r = 0.999 4), whose average recoveries were 98.95%(RSD = 1.94%) and 97.18%(RSD = 3.46%), respectively. The samples from Tibet were clustered into one category, while the others were distributed discretely. The classification results were related to regions. Conclusion:This stable and reliable method could be used for the quality evaluation of G. affine.更多还原