【摘要】 目的 探讨莪术二酮是否通过溶栓作用来抑制大鼠深静脉血栓（DVT）的形成。方法 将25只大鼠随机分为5组：正常对照组、假手术组、模型组、莪术二酮低（60mg/kg）和高（120 mg/kg）剂量组，每组5只。采用下腔静脉结扎模型，给药1周后腹主动脉取血于枸橼酸钠抗凝管内，检测各组大鼠纤维蛋白降解产物（FDP）、D-二聚体（D-D）、纤溶酶原激活物抑制因子（PAI）-1、纤溶酶原（PLG）等纤溶指标；检测下腔静脉血栓重量和重量长度比值；苏木素-伊红（HE）染色法检测大鼠下肢静脉血栓的形成；Western Blot实验检测下腔静脉组织核因子（NF）-κB蛋白表达。结果 各组大鼠血浆FDP水平差异无统计学意义（P>0.05）；与模型组比较，莪术二酮给药组大鼠血浆D-D水平含量升高，PAI-1、PLG水平含量下降，且高剂量莪术二酮给药组的纤溶作用优于低剂量给药组（P<0.05）；与模型组相比，莪术二酮给药组血栓重量/重量长度比、血栓重量、NF-κB蛋白表达量降低（P<0.05）。结论 莪术二酮可能通过影响纤溶调节因子，从而抑制大鼠DVT的形成。更多还原

【Abstract】 Objective To investigate whether curdione inhibits the formation of deep vein thrombosis(DVT) in rats through thrombolytic effects.Methods Twenty-five rats were randomly divided into five groups,the normal control group,the sham operation group,the model group,the low dose group of curdione(60 mg/kg) and the high dose group of curdione(120 mg/kg),with five rats in each group.Using the inferior vena cava ligation model,blood was taken from the pulmonary aorta in a sodium citrate anticoagulant tube one week after drug administration.The coagulation and fibrinolytic indexes Fibrin Degradation Products,(FDP),D-dimer(D-D),plasminogen activator inhibitor(PAI)-1 and plasminogen(PLG) were detected in each group;the thrombus weight/weight-length ratio was detected;and the formation of venous thrombus in the lower limbs of rats was detected by hematoxylin-eosin(HE) staining nuclear factor;NF-κB protein expression in inferior vena cava tissues was dectected by Western Blot assay.Results There was no significant difference in plasma FDP levels among groups of rats(P>0.05);compared with the model group,the plasma FDP and D-D levels of rats in each dosing group were increased,the plasma PAI-1 and PLG levels were elevated,and the fibrinolytic effect was better in the group with high dose of curdione administration than in the group with low dose of curdione administration(P<0.05);compared with the model group,the thrombus weight/weight-length ratio,the thrombus mass and NF-κB protein expression in the curdione dosing group were decreased.Conclusion Curdione can inhibit the formation of DVT in rats by influencing the fibrinolytic regulators.更多还原