【摘要】 目的：研究针刀对兔膝骨关节炎(KOA)模型中骨骼肌纤维化和胶原沉积的影响。方法：将18只兔子随机分为对照组、KOA组和KOA+针刀(Apo)组(每组6只)。KOA和Apo组通过改良的Videman法造模6周。造模成功后，Apo组在股内侧肌、股外侧肌、股直肌、股二头肌和鹅足囊腱处每周针刀干预一次，持续3周。记录所有动物的行为，取股直肌组织，使用Masson染色和透射电子显微镜观察股直肌组织形态变化。Western blot和RT-PCR检测转化生长因子-β1(TGF-β1)、Smad3、Smad7、Ⅰ型胶原(Col-Ⅰ)和Ⅲ型胶原(Col-Ⅲ)的蛋白和mRNA的表达。结果：形态学分析显示，与KOA组相比，Apo组改善了股直肌的微结构并降低了胶原容积分数(P=.034)。针刀通过调节纤维化相关指标的蛋白和mRNA的表达，抑制了异常的胶原沉积，从而减轻骨骼肌纤维化。Western blot和RT-PCR分析显示，Apo组的Col-Ⅰ和Col-Ⅲ蛋白水平显著低于KOA组(P <.01;P <.01)，同样Col-Ⅰ和Col-Ⅲ的m RNA水平也显著降低(P=.0031;P=.0046)。与KOA组相比，Apo组的TGF-β1和Smad 3蛋白水平显著降低(P <.01;P <.01),TGF-β1和Smad 3的mRNA水平也显著降低(P=.0007;P=.0011)。相反，Apo组Smad 7的蛋白和mRNA水平显著高于KOA组(P <.01;P=.0271)。结论：针刀通过抑制骨骼肌中胶原沉积，减轻骨骼肌纤维化，从而发挥延缓KOA进展的作用，该过程通过TGF-β/Smad通路而实现。更多还原

【Abstract】 Objective:To investigate the effects of acupotomy on skeletal muscle fibrosis and collagen deposition in a rabbit knee osteoarthritis(KOA) model.Methods:Rabbits(n = 18) were randomly divided into control,KOA,and KOA + acupotomy(Apo) groups(n = 6).The rabbits in the KOA and Apo groups were modeled using the modified Videman’s method for 6 weeks.After modeling,the Apo group was subjected to acupotomy once a week for 3 weeks on the vastus medialis,vastus lateralis,rectus femoris,biceps femoris,and anserine bursa tendons around the knee.The behavior of all animals was recorded,rectus femoris tissue was obtained,and histomorphological changes were observed using Masson staining and transmission electron microscopy.The expression of transforming growth factor-β1(TGF-β1),Smad 3,Smad 7,fibrillar collagen types Ⅰ(Col-Ⅰ)and Ⅲ(Col-Ⅲ) was detected using Western blot and real-time polymerase chain reaction(RT-PCR).Results:Histological analysis revealed that acupotomy improved the microstructure and reduced the collagen volume fraction of rectus femoris,compared with the KOA group(P =.034).Acupotomy inhibited abnormal collagen deposition by modulating the expression of fibrosis-related proteins and mRNA,thus preventing skeletal muscle fibrosis.Western blot and RT-PCR analysis revealed that in the Apo group,Col-Ⅰ,and Col-Ⅲ protein levels were significantly lower than those in the KOA group(both P <.01),same as Col-Ⅰ and Col-Ⅲ mRNA levels(P =.0031;P =.0046).Compared with the KOA group,the protein levels of TGF-β1 and Smad 3 were significantly reduced(both P <.01),as were the mRNA levels of TGF-β1 and Smad 3(P =.0007;P =.0011).Conversely,the levels of protein and mRNA of Smad 7 were significantly higher than that in the KOA group(P <.01;P =.0271).Conclusion:Acupotomy could alleviate skeletal muscle fibrosis and delay KOA progress by inhibiting collagen deposition through the TGF-β/Smad pathway in the skeletal muscle of KOA rabbits.更多还原