【摘要】 目的 探寻丹参酮ⅡA对脓毒症肺血管内皮细胞损伤的保护作用。方法 采用盲肠结扎穿孔术构建脓毒症肺损伤小鼠模型，分为6组：假手术组、模型组、丹参酮1组（10 mg/kg）、丹参酮2组（30 mg/kg）、丹参酮3组（50 mg/kg）、丹参酮4组（100 mg/kg）。药物干预24 h后检测肺组织病理变化、肺微血管通透性、肺组织湿/干重比、白细胞计数以及炎症因子等各项指标，假手术组仅行盲肠探查术。结果 丹参酮各组较模型组小鼠肺组织损伤及评分均减轻（P <0.05）。与模型组比较，丹参酮各组小鼠肺组织Evans Blue含量、肺组织湿/干比明显降低（P <0.05）,BALF白细胞计数、蛋白含量、炎症因子明显减少（均P <0.05），且呈剂量依赖性，其中丹参酮2组、丹参酮3组和丹参酮4组之间，肺组织病理损伤、Evans Blue含量、肺组织湿/干比以及肺泡灌洗液中白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)表达水平差异无统计学意义（均P>0.05）。结论 丹参酮ⅡA能够剂量依赖性地降低脓毒症所致的肺微血管内皮细胞损伤，从而改善肺损伤；30 mg/kg剂量的丹参酮ⅡA即可达到最佳效果更多还原

【Abstract】 Objective:To explore the protective effect of TanshinoneⅡA(TanⅡA)on endothelial cell injury in septic pulmonary vasculature. Methods:A mouse model of septic lung injury was constructed using cecal ligation and puncture(CLP). Mice were divided into six groups:sham operation group,model group,experimental group 1[CLP+ 10 mg/kg TanⅡA],experimental group 2[CLP+30 mg/kg TanⅡA],experimental group 3[CLP+50 mg/kg TanⅡA],and experimental group 4[CLP+100 mg/kg TanⅡA]. After 24 hours of treatment,various indicators such as lung tissue pathology,pulmonary microvascular permeability,lung tissue wet/dry weight ratio,white blood cell count,and inflammatory factors were measured. The sham group underwent only cecal exploration. Results:The experimental groups showed reduced lung tissue injury and scores compared with the model group(P < 0.05).Compared with the model group,the experimental groups exhibited significantly lower Evans Blue content and lung tissue wet/dry weight ratio(P < 0.05). Compared with the model group,the experimental groups had significantly reduced white blood cell count,protein content,and inflammatory factors in bronchoalveolar lavage fluid,with statistically significant differences(all P < 0.05). There were no significant differences in lung tissue pathological damage,Evans Blue content,lung tissue wet/dry weight ratio,and the expression levels of IL-6,TNF-α,IL-1β,and other inflammatory factors in bronchoalveolar lavage fluid among experimental group 2,experimental group 3,and experimental group 4(all P > 0.05). Conclusion:TanⅡA can dose-dependently reduce endothelial cell injury in septic pulmonary microvasculature,thus improving lung injury,and 30 mg/kg TanⅡA can achieve the best effect.更多还原