【摘要】 目的 基于胃黏膜菌群探讨胃复春治疗胃癌前病变的功效机制。方法 临床招募37例胃黏膜肠化生(gastric intestinal metaplasia,GIM)患者作为治疗组,胃复春治疗4周,31例健康志愿者为对照组。基于16S r RNA基因高通量测序检测胃黏膜菌群。结果 37例GIM患者经胃复春治疗后,23例患者肠化生未检出,5例患者GIM等级下降,肠化生等级改善有效率75.7%。与治疗前相比,GIM患者治疗后胃黏膜菌群丰富度指数(Ace,Chao,Observed OTUs)显著升高(P<0.01),但菌群多样性指数(Shannon)无显著变化(P>0.05)。线性判别分析发现,16个胃黏膜菌属(Acinetobacter、Fusobacterium、Leptotrichia等)富集在胃复春治疗组,胃复春治疗增强4个KEGG菌群预测功能(膜转运、ABC转运蛋白、脂类代谢、脂肪酸代谢)。结论 胃复春治疗促进GIM患者胃菌群丰富度和脂质代谢功能,可能体现了胃复春活血化瘀功效机制。更多还原

【Abstract】 Objective The study aims to explore the effect and mechanisms of Weifuchun on gastric precancerous lesions based on gastric microbiota. Methods Thirty-seven patients with gastric intestinal metaplasia(GIM) were recruited as treatment group, who received the treatment of Weifuchun for four weeks. Thirty-one healthy individuals were selected as the control group. Gastric microbiota was sequenced based on 16S r RNA gene high-throughput sequencing. Results After treatment with Weifuchun in the 37 GIM patients, no GIM was detected in 23 patients, the grades of GIM decreased in 5patients, and the total effective rate of downgrading GIM was 75. 7%. After treatment of Weifuchun in GIM patients, the richness indices(Ace, Chao, Observed OTUs) of microbiota were significantly increased(P<0. 01), but the diversity index(Shannon) was not significantly affected by Weifuchun treatment(P>0. 05). Linear discriminant analysis(LDA)showed that 16 bacterial genera(Acinetobacter, Fusobacterium, Leptotrichia, etc.) were enriched in GIM patients after Weifuchun treatment. The KEGG predicting function showed that membrane transport, ABC transporters, lipid metabolism and fatty acid metabolism pathway were enriched in the GIM patients with Weifuchun treatment. Conclusion Weifuchun treatment significantly increased the abundance of gastric microflora and the predictive function lipid metabolism in GIM patients, which might represent the blood-activating and stasis-eliminating function.更多还原