【摘要】 以体外培养奶牛子宫内膜组织为研究对象，运用ELISA、荧光定量PCR、HE染色和免疫荧光法分析了内源性和外源性前列腺素D₂（prostaglandin D₂,PGD₂）在大肠杆菌（Escherichia coli,E.coli）感染奶牛子宫内膜组织过程中对细胞因子（IL-1β、IL-6、TNF-α）和损伤相关因子（HMGB-1）表达的调控作用。结果显示，与空白对照组相比较，E.coli感染组奶牛子宫内膜组织中COX-2和H-PGDS的基因表达显著升高（P<0.001）;PGD₂分泌量也显著上升（P<0.001）;内源性PGD₂抑制剂（H-PGDS inhibitor 1、HQL-79）能够显著抑制E.coli感染奶牛子宫内膜组织中促炎细胞因子（IL-1β、IL-6、TNF-α）的分泌、损伤相关因子（HMGB-1）的表达并减轻了组织损伤；外源性添加PGD₂后可显著促进E.coli感染奶牛子宫内膜组织中促炎细胞因子的分泌，HMGB-1的表达及组织损伤程度明显增加。结果表明，PGD₂能够通过调控E.coli感染奶牛子宫内膜组织的过程促炎细胞因子和损伤相关因子的表达，进而加剧了由细菌感染引起的组织损伤。更多还原

【Abstract】 The aim of this study is to investigate the effect of endogenous and exogenous prostaglandin D2(PGD2) on the expression of cytokines(IL-1β,IL-6,TNF-α)and injury related factors(HMGB-1) in the endometrium of cows infected with Escherichia coli(E.coli).The results showed that compared with the blank control group, the gene expression of COX-2 and H-PGDS in the endometrium of cows infected with E.coli was significantly increased(P<0.001).The secretion of PGD2 also significantly increased(P<0.001).Endogenous PGD2 inhibitors(H-PGDS Inhibitor 1,HQL-79) can significantly inhibit pro-inflammatory cytokines)in the endometrium of cows infected with E.coli(IL-1β,IL-6,TNF-α).The secretion and expression of injury related factors(HMGB-1)in mice reduced tissue damage.The exogenous addition of PGD2can significantly promote the secretion of pro-inflammatory cytokines in the endometrium of cows infected with E.coli,and significantly increase the expression of HMGB-1and the degree of tissue damage.The above results indicate that PGD2can regulate the expression of pro-inflammatory cytokines and injury related factors in the process of E.coli infection of bovine endometrial tissue,thereby exerting a regulatory effect on tissue damage caused by bacterial infection.更多还原