【摘要】 结合形态特征和分子系统发育分析，将采自云南省红河哈尼族彝族自治州屏边苗族自治县腐木上的野生木耳鉴定为泰国木耳（Auricularia thailandica）。经组织分离获得纯培养菌株，并首次对中国采集的泰国木耳开展固体培养、液体培养和驯化栽培试验。试验结果表明，固体培养时的最适配方为可溶性淀粉20.0 g·L^-1、豆饼粉2.0g·L^-1、氯化钙3.0 g·L^-1、琼脂粉18.0 g·L^-1,pH为7，培养温度为32℃。液体培养时的最适配方为可溶性淀粉20.0 g·L^-1、蛋白胨2.0 g·L^-1、磷酸氢二钾3.0 g·L^-1,pH为6，培养温度为26℃。栽培试验结果显示其原基分化较快，耳片一致性较好，子实体肥厚、耳形规整，产孢面与野生子实体相同，有明显皱褶，有非常大的应用价值，可作为新种质资源开发和利用。更多还原

【Abstract】 Auricularia-like species growing on decaying wood in Pingbian Miao Autonomous County, Honghe Hani and Yi Autonomous Prefecture, Yunnan Province, were identified as Auricularia thailandica based on morphological characters and molecular phylogenetic analysis. Pure strain was obtained by tissue isolation, and solid-state culture, liquid-state culture, domestication cultivation of this specie collected in China was carried out for the first time. The experimental results showed that under solid-state conditions, the optimal formula was 20.0 g·L^-1soluble starch, 2.0 g·L^-1soybean meal, 3.0 g·L^-1calcium chloride,18.0 g·L^-1agar powder, the pH was 7 and the culture temperature was 32 ℃. Under liquid-state conditions, the optimal formula was 20.0 g·L^-1soluble starch, 2.0 g·L^-1peptone, 3.0 g·L^-1dipotassium phosphate, the pH was 6 and the culture temperature was 26 ℃. The results of cultivation experiment showed that it’s primordium differentiated quickly, the ear segments were consistent, the fruit bodies was thick, the ear shape was regular, and the sporulation surface was the same as the wild fruit bodies,with obvious folds, which had great application value and could be used as new germplasm resources.更多还原