【摘要】 在辣椒叶片中分离到一株对瓜类细菌性果斑病菌具有显著拮抗作用的菌株ZY1。通过形态特征观察、Biolog测定及多基因系统发育分析，鉴定菌株ZY1为贝莱斯芽胞杆菌Bacillusvelezensis。对菌株ZY1的生物学测定结果表明，菌株ZY1在pH 5～9和NaCl浓度2%～7%条件下均能够生长，25℃～40℃为最适生长温度。通过抑菌实验分析，拮抗菌ZY1对瓜类细菌性果斑病I组菌株pslb65和II组菌株Aac5均具有较强的抑菌作用。酶学试验结果表明，菌株ZY1能够产生纤维素酶、果胶酶、蛋白酶和淀粉酶等代谢物质。同时，该菌株还具有解磷和产氨能力。本文首次报道了一株对瓜类细菌性果斑病菌具有抗菌作用的贝莱斯芽胞杆菌ZY1，该菌为瓜类细菌性果斑病的生物防治以及土壤改良提供了新的微生物资源。更多还原

【Abstract】 The strain ZY1 was isolated from pepper leaves, which has a significant antagonistic effect on bacterial fruit blotch. Based on morphological observation, Biolog analysis, and multi gene phylogenetic analysis,strain ZY1 was identified as Bacillus velezensis. To evaluate the stress resistance of strain ZY1, its growth status was studied under different pH, NaCl concentration, and temperature. The results showed that strain ZY1 growed rapidly and has strong stress resistance. It could grow normally under conditions of pH 5-9, NaCl concentration of 2%-7%, and 25 ℃-40 ℃, and the growth of the strain was significantly inhibited at 55 ℃. The antibacterial experiment results showed that strain ZY1 had strong antibacterial effects on both group I and group II strain Aacs. The enzymatic test results showed that strain ZY1 could produce metabolites such as cellulase, pectinase,protease, and amylase. At the same time, the strain also had the ability to hydrolyze phosphorus and produce ammonia. This article reported for the first time a strain of Bacillus velezensis, which had antibacterial activity against bacterial fruit blotch. This strain ZY1 provided a new microbial resource for the biological control and soil improvement.更多还原