【摘要】 亚洲小车蝗是草原上重要的害虫之一，本文研究了3种植物源化合物瑞香狼毒根石油醚提取物、百里香酚和印楝素对亚洲小车蝗3龄蝗蝻的生物活性及影响机制。结果表明，3种植物源化合物对亚洲小车蝗的毒性表现为：拒食作用>胃毒作用>触杀作用。48 h时瑞香狼毒根石油醚提取物、百里香酚和印楝素对亚洲小车蝗3龄蝗蝻拒食活性的拒食中浓度(AFC₅₀)分别为433.97 mg/L、183.35 mg/L和86.47 mg/L，胃毒活性的致死中浓度(LC₅₀)分别为7976.33 mg/L、269.65 mg/L、341.07 mg/L，触杀作用相对较小。3种植物源化合物对亚洲小车蝗3龄蝗蝻离体腺苷三磷酸酶（ATPase）和乙酰胆碱酯酶（ACh E）活性均有抑制作用，且随着药剂浓度增大抑制作用增强，抑制率不超过50%。3种化合物处理亚洲小车蝗3龄蝗蝻48 h后，亚洲小车蝗体内谷胱甘肽-S-转移酶（GSTs）和细胞色素P450活性升高，羧酸酯酶（CarE）活性受到抑制。研究结果表明，这3种植物源化合物对亚洲小车蝗都具有一定的杀灭作用，拒食作用最强，未来在亚洲小车蝗的防控上可进一步开发利用。更多还原

【Abstract】 Oedaleus decorus asiaticus is one of the important pests on grassland.The mechanism and the biological activity of the petroleum extract from Stellera.chamaejasme roots,azadirachtin and thymol to the third instar of O.decorus asiaticus were measured in this paper.The results showed that the toxicity of the three plant derived compounds to O.decorus asiaticus was antifeedant activity>stomach activity>contact activity.The median antifeedant concentration (AFC₅₀) of petroleum ether extract from S.chamaejasme root,thymol and azadirachtin on the third instar of O.decorus asiaticus was 433.97 mg/L,183.35 mg/L and 86.47 mg/L respectively,and the median lethal concentration (LC₅₀) of stomach activity was 7976.33 mg/L,269.65 mg/L and 341.07 mg/L respectively at 48h.The contact activities of the three plant derived compounds were small relatively.The activities of ATPase and AChE in vitro of the third instar of O.decorus asiaticus were inhibited by the three plant derived compounds,and the inhibition increased with the increasing of the concentrations,but the inhibition rate was not more than 50%.The activity of GSTs and cytochrome P450 of the third instar of O.decorus asiaticus increased,but the activity of CarE was inhibited after treatment of 48 h.The results showed that O.decorus asiaticus could be controlled by three plant derived compounds,and antifeedant activity was the most.The three plant derived compounds can be developed in controling O.decorus asiaticus in the future.更多还原