【摘要】 目的 了解福州城市公共绿地螺类感染广州管圆线虫情况，为制定广州管圆线虫病控制策略提供依据。方法 2021—2023年5～11月在福州市区各大公园、住宅小区、城市道路旁等公共绿地采集褐云玛瑙螺和福寿螺，匀浆法收集广州管圆线虫Ⅲ期幼虫并进行形态学鉴定。提取阳性螺广州管圆线虫基因组DNA进行PCR扩增核糖体大亚基rRNA基因，测序后进行BLAST比对。结果 共采集213只褐云玛瑙螺，感染率为20.2%(43/213)，平均感染度235.8条/螺（10 139/43）；共采集507只福寿螺，感染率为16.0%(81/507)，平均感染度为43.2条/螺（3 501/81）。BLAST比对结果显示，PCR扩增产物测序结果与GenBank数据库中广州管圆线虫基因序列（GenBank登录号：AB684364.1）的一致性为98.2%。213只褐云玛瑙螺的体质量中位数为36 g，体质量在36 g以下和以上的感染率分别为11.2%(12/107)和29.2%(31/106)，感染率差异有统计学意义（χ~2=10.74,P<0.05）。体质量大的福寿螺感染率高于体质量较小者，感染率差异有统计学意义（χ~2=181.03,P<0.05）。结论 福州城市公共绿地存在广州管圆线虫阳性的褐云玛瑙螺和福寿螺，生食、半生食螺肉或接触被褐云玛瑙螺分泌物污染的物体存在被感染风险。更多还原

【Abstract】 Objective To investigate the infection of Angiostrongylus cantonensis in snails in public greenbelts of Fuzhou, so as to provide the scientific basis for the formulation of the angiostrongyliasis control.Methods A. fulica and P. canaliculata were randomly collected from a public green land in public parks,residential areas, urban roads and other public green areas during May to November of 2021 and 2023. The A. cantonensis stage Ⅲ larvae were collected by homogenizing method and identified by morphology. A. cantonensis rRNA gene was amplified by PCR and the sequence was compared by BLAST analysis.Results A total of 213 A. fulica were collected, 20. 2%(43/213) A. fulica were infected with A. cantonensis. The average infection degree was 235. 8(10 139/43)/snail. A total of 507 P. canaliculata were collected, 16. 0%(81/507) P. canaliculata were infected with A. cantonensis. The average infection degree was 43. 2(3 501/81)/snail. The BLAST results showed that the consistency between the PCR product sequencing results and the reference sequences of A. cantonensis in the GenBank(Accession number: AB684364. 1) was 98. 2%. The median body weight of 213 A. fulica was 36g, and the infection rates of A. fulica with body weight below 36g and above were 11. 2%(12/107) and 29. 2%(31/106),and there was significant difference in infection rate （χ~2=10. 74, P<0. 05）. The infection rate of P. canaliculata with larger body mass is higher than that of larger body mass with smaller body mass, and there was a significant difference among snails with different body masses（χ~2=181. 03, P<0. 05）.Conclusion The results suggested that there are A. fulica and P. canaliculata infected with A. cantonensis in public greenbelts of Fuzhou city, This will be provide a infection risk upon eating the raw or partially cooked or touching the objects contaminated with A. fulica secretions.更多还原