【摘要】 目的 了解2023年江西省市售水产品中副溶血性弧菌的污染水平、耐药性及遗传特征情况。方法2023年采集江西省市售的212份水产品，分析不同季度和不同种类副溶血性弧菌检出情况，并进行ST型、毒力基因、耐药基因和抗生素敏感性检测。结果 212份样品共分离出45株副溶血性弧菌，总检出率为21.23%。海水产品和淡水产品检出率分别为26.67%和15.89%，检出率差异无统计学意义（χ~2=3.682,P>0.05）。第三季度检出率最高为22.62%；甲壳类和贝类检出率最高，均为25.00%。45株分离菌株对头孢唑啉（100.00%）和多黏菌素E(51.11%)具有高耐药性，共发现5株多重耐药菌株，其中3株分离自海水鱼类。45株分离菌株共发现40个STs，其中17个为新发现STs，无优势STs。cgMLST系统发育分析表明45株分离菌株亲缘关系较远。45株分离菌株均携带率tlh和T3SS1基因，仅2株分离菌株携带了trh基因，未检测到tdh和T3SS2基因。5株多重耐药菌株均携带tlh和T3SS1基因，未检测到tdh、trh和T3SS2基因。共检测到13种耐药基因，分别为CARB-20、tet(35)、sul1、sul2、dfrA6、CRP、QnrS2、ANT(2’’)-Ia、ACC(6’)-Ia和tet(E)，耐药基因的携带与耐药表型不完全一致。结论 江西省市售水产品中存在一定程度的副溶血性弧菌污染，副溶血性弧菌间遗传多样性丰富，亲缘关系较远，毒力基因携带率低，存在多重耐药菌株，耐药机制复杂，还有待进一步的研究。更多还原

【Abstract】 Objective To analyze the contamination, drug resistance and genetic characteristics of Vibrio parahaemolyticus isolated from aquatic products in Jiangxi Province in 2023.Methods A total of 212 aquatic products sold in Jiangxi Province in 2023 were collected, and the contamination of Vibrio parahaemolyticus in different seasons and different kinds was analyzed. The STs, virulence gene, resistance gene and antibiotic sensitivity of Vibrio parahaemolyticus were also detected.Results A total of 45 strains of Vibrio parahaemolyticus were detected from 212 samples with a detection rate of 21. 23%. The detection rate of Vibrio parahaemolyticus in marine products and freshwater products were 26. 67% and 15. 89%, respectively, while there was no significant difference in detection rate （χ~2=3. 682, P>0. 05）. The detection rate in the third quarter was highest(22. 62%). The detection rate in crustacea and shellfish were highest,both of which were 25. 00%. The 45 isolates presented highly resistant to cefazolin(100. 00%) and polymyxin E(51. 11%). A total of 5 multiple drug-resistant strains were found, and 3 of which were isolated from marine fish. A total of 40 STs were found in 45 isolates, of which 17 were newly discovered STs, and no dominant STs were found. The phylogenetic analysis of cgMLST showed that the 45 isolates were distantly related. All 45 isolates carried tlh and T3SS1 genes, only 2 isolates carried trh gene, and tdh and T3SS2 genes were not detected. All 5 multiple drug-resistant strains carried tlh and T3SS1genes, and tdh, trh and T3SS2 genes were not detected. A total of 13 drug resistance genes were detected, including CARB-20, tet(35), sul1, sul2, dfrA6, CRP, QnrS2, ANT(2")-Ia, ACC(6’)-Ia and tet(E). The carrying of resistance genes is not completely consistent with the resistance phenotype.Conclusion The aquatic products sold in Jiangxi province were polluted by Vibrio parahaemolyticus at a certain degree. The Vibrio parahaemolyticus strains has rich genetic diversity and presented a distantly related. The carrying rate of virulence gene was low. The multiple drug-resistant strains were found, and the drug resistance mechanism needed to further studied because of its complexity.更多还原