【摘要】 目的 无镁细胞外液建立癫痫放电模型，利用全细胞膜片钳技术，检测仓鼠原代皮质神经元的电生理学特性。方法 采用生后1～2 d新生叙利亚仓鼠，分离大脑皮质培养原代神经元培养至第12天，分别予有镁细胞外液（有镁组）和无镁细胞外液（无镁组）孵育3 h,3 h后均更换为正常孵育液继续培养24 h。利用全细胞膜片钳技术，电压钳模式下记录神经元兴奋性突触后电流（excitatory postsynaptic currents,EPSC），电流钳模式下记录神经元兴奋性突触后电位（excitatory postsynaptic potentials,EPSP）。结果与有镁组相比，无镁组仓鼠原代皮质神经元EPSC[(124.38±75.15)Hz vs.(33.93±22.32)Hz,P<0.001]及EPSP [(37.05±38.37)Hz vs.(5.63±9.52)Hz,P<0.01]的频率升高，且差异有统计学意义；而两组之间EPSC及EPSP的振幅、曲线下面积和半宽度的差异无统计学意义（P>0.05）。结论 无镁处理后仓鼠原代皮质神经元兴奋性升高，仓鼠原代皮质神经元可用于构建癫痫细胞模型。更多还原

【Abstract】 Objective To investigate the electrophysiological characteristics of hamster primary cortical neurons by using the whole-cell patch-clamp recordings in a Mg²⁺-free cell model of epilepsy.Methods The cerebral cortex were isolated for primary neuron culture from neonatal Syrian hamsters 1-2 days after birth.After 12 days,primary neurons were exposed to normal external (Mg²⁺group) or Mg²⁺-free external (Mg²⁺-free group) respectively for 3 hours,followed by a 24-hour incubation in normal culture medium.Excitatory postsynaptic currents （EPSC） and excitatory postsynaptic potentials （EPSP） were recorded in voltage clamp mode and current clamp mode using whole-cell patch-clamp recordings,respectively.Results Compared to Mg²⁺group,the frequency of EPSC[（124.38±75.15） Hz vs.（33.93±22.32） Hz,P<0.001]（P<0.001） and EPSP[（37.05±38.37） Hz vs.（5.63±9.52） Hz,P<0.01]in primary cortical neurons from the Mg²⁺-free group increased significantly.Meanwhile,there were no statistically significant differences in the amplitude,area under the curve and half-width of EPSC and EPSP between the two groups （P>0.05）.Conclusion The present study demonstrates an increase in excitability of primary hamsters cortical neurons after Mg²⁺depletion,suggesting that these neurons can be utilized to use to create cell models of epilepsy.更多还原