【摘要】 目的 了解云南省西部地区宿主动物和媒介蜱感染伯氏疏螺旋体的基因多样性，为进一步掌握该地区莱姆病菌株的特性奠定基础。方法 对采用伯氏疏螺旋体5S～23S rRNA间隔区基因筛查阳性的云南西部地区采集的家畜、小型兽类、蜱虫样本，进行伯氏疏螺旋体鞭毛蛋白基因(FLA基因)和16S rRNA基因的检测，综合分析病原体的基因信息，了解当地流行株的生物学特性及流行特征。结果 共检测301份样本，共检出7种基因型伯氏疏螺旋体，其中宿主动物共检出5种：B.afzelii(阿弗西尼疏螺旋体)、B.garinii(伽氏疏螺旋体)、B.burgdorferi s.s.(狭义伯氏疏螺旋体)、B.japonica(日本疏螺旋体)、B.valaisiana(法雷斯疏螺旋体);媒介蜱检出7种：B.afzelii(阿弗西尼疏螺旋体)、B.garinii(伽氏疏螺旋体)、B.burgdorferi s.s.(狭义伯氏疏螺旋体)、B.japonica(日本疏螺旋体)、B.valaisiana(法雷斯疏螺旋体)、B.sinica(中华基因型伯氏疏螺旋体)及未定种Borrelia sp.。B.afzelii、B.garinii和B.burgdorferi s.s.为优势流行株，占总阳性的85.04%(256/301)。不同地区、不同来源的相同基因型株基因序列存在种内差异，同一地区的宿主和媒介中检出的相同基因型螺旋体具有较高的同源性和较近的遗传进化关系。结论 云南西部地区宿主和媒介感染伯氏疏螺旋体基因型具有较高的多样性特征，存在多种致病基因型，给当地人群的健康造成一定危害，需加强防范。更多还原

【Abstract】 This study was aimed at understanding the genetic diversity of Borrelia burgdorferi infected by host animals and vector ticks in the western region of Yunnan province, to provide a basis to characterize epidemic strains or genotypes in this region. Genomic DNA templates were extracted from livestock, small mammals and tick specimens in western Yunnan province, and Borrelia DNA was identified through nested PCR amplification of the 5S-23S rRNA intergenic spacer region. Positive samples were further tested with 16S rRNA amplification and verified with the flagellin(FLA) gene. Five genotypes of B. burgdorferi sensu lato were detected in the host animals: B. afzelii, B. garinii, B. burgdorferi s. s., B. japonica and B. valaisiana. Seven genotypes were found in vector ticks: B. afzelii, B. garinii, B. burgdorferi s. s., B. japonica, B. valaisiana, B. sinica and Borrelia sp. Moreover, B. afzelii, B. garinii and B. burgerdorferi s. s. were found to be the dominant genotypes, accounting for 85.04%(256/301) of all positive samples. Intraspecific variations were observed in the same genotype of B. burgdorferi from different regions and specimen sources. B. burgdorferi detected in hosts and vectors in the same region showed high homology and close phylogenetic relationships. The high genetic diversity of B. burgdorferi in the hosts and vectors from western Yunnan, and their pathogenicity to humans, indicate potential health hazards to local residents. Prevention and control of Lyme borreliosis must urgently be strengthened in these regions.更多还原