【摘要】 目的 探讨血浆可溶性平足蛋白（plasma soluble podoplanin,sPDPN）在结直肠癌（coloretal cancer,CRC）患者中的表达及其临床意义。方法 获取TCGA-READ、TCGA-COAD数据集，比较CRC组织和癌旁组织中PDPN mRNA的表达水平，并探索PDPN mRNA的表达与CRC患者预后的关系。回顾性收集2020年11月至2022年12月期间于徐州医科大学第二附属医院普外科接受手术的CRC患者85例（CRC组），收集同期来院体检的100例健康自愿者作为对照组，比较2组研究对象的血浆sPDPN的表达水平，并探索sPDPN表达和CRC患者临床病理特征的关系。同时绘制sPNPD、糖类抗原199(carbohydrate antigen 199,CA199)和癌胚抗原（carcinogenicity antigen,CEA）预测CRC的ROC曲线，并采用logistic回归模型探索sPDPN对CRC的预测价值。结果 TCGA数据集结果表明，与癌旁组织/配对癌旁组织比较，PDPN mRNA在CRC组织中表达上调（P<0.05）;PDPN mRNA高表达组患者的预后优于低表达组患者（P=0.045）。CRC组sPDPN的表达水平为（3.50±1.77）ng/mL，高于对照组的（1.95±0.46）ng/mL,P<0.01。临床分期Ⅲ+Ⅳ期CRC患者的sPDPN表达水平高于Ⅰ+Ⅱ期者（P=0.026）,N1～2期CRC患者的sPDPN表达水平高于N0期者（P=0.049）。与CA199和CEA比较，sPDPN诊断CRC的曲线下面积值最高，为0.882(0.832,0.932)。多因素logistic回归分析结果显示，sPDPN高水平是CRC的危险因素[OR=14.769,95%CI(5.867,37.174),P<0.001]。结论 sPDPN在CRC患者血浆中高表达，对CRC的诊断、临床分期和淋巴结转移评估有一定的临床应用价值。更多还原

【Abstract】 Objective To explore the expression and clinical significance of plasma soluble podoplanin(sPDPN in patients with colorectal cancer(CRC). Methods TCGA-READ, TCGA-COAD datasets were obtained to compare expression level of PDPN mRNA in CRC tissues and adjacent tissues, and to explore the relationship between expression of PDPN mRNA and the prognosis of CRC patients. A total of 85 CRC patients(CRC group) underwent surgery in the Second Affiliated Hospital of Xuzhou Medical University from November 2020 to December 2022, and 100 healthy volunteers from the hospital were collected as a control group to detect the expression levels of plasma sPDPN of the two groups, and exploring the relationship between sPDPN expression and the clinicopathological characteristics of the CRC patients. The ROC curves of sPNPD, sugar antigen 199(CA199) and carcinoembryonic antigen(CEA) were drawn simultaneously, and the predictive value of sPDPN for CRC was explored by logistic regression model. Results The results of TCGA dataset showed that expression level of PDPN mRNA was upregulated in CRC tissues compared with adjacent tissues/paired adjacent tissues(P<0.05), and patients with high expression level of PDPN mRNA had better prognosis than low expression patients(P=0.045). The expression level of sPDPN was(3.50±1.77) ng/mL in CRC group,which was higher than(1.95±0.46) ng/mL of the control group(P<0.01). The expression level of sPDPN was higher in CRC patients at Ⅲ+Ⅳ clinical stage(P=0.026) and N1–2 stage(P=0.049). Compared with CA199 and CEA, sPDPN had the highest area uncer curve value of 0.882(0.832, 0.932), and was an risk predictor of CRC [OR=14.769, 95%CI(5.867,37.174), P<0.001]. Conclusion The sPDPN is highly expressed in the plasma of CRC patients and has a certain clinical utility for diagnosis of CRC, and evaluation of clinical staging and lymph node metastasis.更多还原