【摘要】 目的 采用超高效液相色谱-四极杆-静电场轨道阱高分辨质谱（UPLC-Q-Orbitrap-HRMS）技术分别对以水和50%甲醇溶液为溶剂提取的葛根-丹参药对样品进行化学成分分析。方法 采用Thermo Scientific Hypersil GOLD C₁₈色谱柱（150 mm×2.1 mm，1.9 μm），以0.1%甲酸水-0.1%甲酸乙腈溶液为流动相进行梯度洗脱，流速为0.3 mL·min^-1，柱温为30℃。采用加热电喷雾离子源（H-ESI），正负离子检测模式。结果 从水提和50%甲醇醇提样品溶液中均鉴定出104个化合物，其中103个化合物为共有成分，各有1个差异性成分，分别为水提样品中的刺槐苷与50%甲醇醇提样品中的降丹参酮（或其同分异构体）。共鉴定出的105个化合物，包括黄酮类成分36个、苯丙素类成分20个、萜类成分24个和其他类成分25个。结论 本研究首次采用UPLC-Q-Orbitrap-HRMS技术对葛根-丹参药对的主要成分进行鉴定，可为葛根-丹参药对的药效成分研究提供科学依据。更多还原

【Abstract】 Objective To analyze the chemical components of Gegen-Danshen herb pair extracted with water and 50% methanol solution by UPLC-Q-Orbitrap-HRMS.Methods Thermo Scientific Hypersil GOLD C₁₈ column （150 mm×2.1 mm,1.9 μm） was used for gradient elution with 0.1% formic acid water-0.1% formic acid acetonitrile solution as the mobile phase.The flow rate was 0.3 mL·min^-1 and the column temperature was 30℃.A heated electrospray ionization （H-ESI） source was used for HRMS analysis in both positive and negative ion modes.Results In both water and 50% methanol extract samples,104 compounds were tentatively identified:103 common components,and one differentials component,namely acacetin in the water extract and nortanshinone （or its isomers） in 50% methanol extract.Among the identified 105 compounds,there were 36 flavonoids,20 phenylpropanoids,24 terpenoids and 25 other components.Conclusion The main components of Gegen-Danshen herb pair are characterized by UPLC-Q-Orbitrap-HRMS for the first time,which provides reference for the discovery of bioactive components in Gegen-Danshen herb pair.更多还原