【摘要】 为建立一套能够快速准确鉴定四倍体大白菜倍性的流式细胞术应用方法，以12种已知倍性的蔬菜作物验证流式细胞仪倍性检测结果，同时以二倍体大白菜‘冀白4号’和四倍体大白菜‘多维462’、‘多505’和‘多育2号’为材料，对不同时期、不同部位取材并进行倍性鉴定，最终筛选出合适的取样时期与部位。结果表明，12种已知倍性的蔬菜验证了本研究所用流式细胞仪鉴定结果的准确性与可靠性；‘冀白4号’、‘多维462’、‘多505’和‘多育2号’在子叶期取幼嫩子叶的倍性检测结果均与已知倍性相符。因此，子叶期的幼嫩子叶为大白菜鉴定倍性取材的最佳时期与部位。更多还原

【Abstract】 In order to establish a set of flow cytometry application methods that can rapidly and accurately identify the ploidy of tetraploid Chinese cabbage, 12 vegetable crops with known ploidy were used to verify the results of ploidy detection by flow cytometry. At the same time, diploid Chinese cabbage‘Jibai 4’and tetraploid Chinese cabbage‘Duowei 462’,‘Duo 505’and‘Duoyu 2’were selected as materials, and the ploidy identification was carried out in different periods and parts of the plant, so as to screen out the suitable sampling periods and parts. The results showed that 12 vegetables with known ploidy verified the accuracy and reliability of the flow cytometry results used in this experiment; the ploidy detection results of young cotyledons taken from‘Jibai 4’,‘Duowei 462’,‘Duo 505’and‘Duoyu 2’during the cotyledon stage were consistent with known ploidy. Therefore, the young cotyledons at the cotyledon stage are the best time and place for ploidy sampling in Chinese cabbage.更多还原