【摘要】 本研究旨在鉴定和分析锦州某猪场病死猪脏器中分离出的一株病原菌，以期为该细菌病的临床诊治提供科学依据。通过LB琼脂培养进行革兰氏染色，BP特殊培养基培养、药物敏感性试验、16S rDNA测序及小鼠致病性试验，并制作病理组织切片进行观察。由革兰氏染色镜检结果可知该菌为革兰氏阳性球菌；BP培养皿可见黑色菌落生长及菌落周边透明圈；药敏试验表明该菌对恩诺沙星、氧氟沙星、阿米卡星和卡那霉素非常敏感；经16S rDNA测序，该菌和金黄色葡萄球菌16S rDNA片段相似性高；镜检观察病理切片，可见各组织脏器有明显病理损害。该猪场致病菌经分离鉴定为金黄色葡萄球菌，对部分抗生素产生耐药，小鼠攻毒试验具有一定的致病性。更多还原

【Abstract】 This study aims to investigate a strain of pathogenic bacteria, isolated from the organs of dead pigs on a Jinzhou pig farm. The research encompasses bacterial isolation and identification, drug sensitivity tests, and clinical diagnosis and treatment implications. Gram staining was conducted using LB agar culture, followed by BP special medium culture. Drug sensitivity testing, 16S rDNA sequencing, and mouse pathogenicity testing were also performed. Pathological tissue sections were prepared for microscopic observation. Gram staining microscopy results indicated that the bacterium was Gram-positive cocci. The BP Petri dish revealed black colony growth with a transparent ring around it. Drug sensitivity tests demonstrated high sensitivity to enrofloxacin, ofloxacin,amikacin, and kanamycin. 16S rDNA sequencing revealed a high similarity between the 16S rDNA fragments of this bacterium and Staphylococcus aureus. Microscopic examination of pathological sections revealed significant pathological damage in various tissues and organs. The causative organism on this pig farm was identified as Staphylococcus aureus, which exhibited resistance to certain antibiotics and demonstrated some pathogenicity in themouseattacktest.更多还原