【摘要】 该研究从原始森林腐木的土壤中取样，筛选产β-葡萄糖苷酶菌株，对其产β-葡萄糖苷酶能力进行测定，并对高产菌株进行形态特征、分子生物学鉴定及酶学性质研究。结果表明，筛选得到13株产β-葡萄糖苷酶菌株，复筛得到1株高产β-葡萄糖苷酶的菌株PX14A，其β-葡萄糖苷酶酶活为4.9 U/m L。菌株PX14A被鉴定为枯草芽孢杆菌（Bacillus subtilis）。酶学性质研究结果表明，菌株PX14A粗酶液的β-葡萄糖苷酶在65℃以下稳定，最适反应温度为60℃，在pH值4.0～7.5较稳定，最适反应pH值为6.5，浓度为1 mmol/L的Mg²⁺和Fe²⁺对酶活性有较好的激活作用。结果显示该菌株具有良好应用前景。更多还原

【Abstract】 β-glucosidase-producing strains were selected from the soil of decayed trees in primitive forests, and the β-glucosidase production ability was measured. The morphological characteristics, molecular biological identification and enzymatic properties of the high-yield strains were studied.The results showed that 13 β-glucosidase-producing strains were screened, a high-yield glucosidase strain PX14A was obtained through re-screening,and the β-glucosidase activity was 4.9 U/ml. The strain PX14A was identified as Bacillus subtilis. The results of enzymatic properties research showed that the β-glucosidase of the strain PX14A crude solution was stable below 65 ℃, the optimal reaction temperature was 60 ℃, the enzyme was stable at pH 4.0-7.5, and the optimal reaction p H value was 6.5. Mg²⁺ and Fe²⁺ with a concentration of 1 mmol/L had a good activating effect on enzyme activity. The results indicated that the strain PX14A had good application prospects.更多还原