【摘要】 为快速识别葡萄酒中是否添加人工合成着色剂并准确定量，该实验样品采用乙酸铵溶液稀释、8 000 r/min离心后直接过滤法进行前处理，梯度洗脱、Zorbax SB-C18柱分离、多波长检测（288 nm、425 nm、515 nm、620 nm），建立了高效液相色谱法（HPLC）同时检测葡萄酒中11种人工合成着色剂的方法。结果表明，该方法前处理简单，各着色剂最大吸收波长检测无杂质干扰、灵敏度高、检出限（0.05 mg/L）、定量限（0.15 mg/L）低，在质量浓度0.20～50 mg/L范围内线性关系良好（R2≥0.999 4），方法精密度试验结果的相对标准偏差（RSD）为1.86%～2.61%，加标回收率为93.0%～103.0%。适用于葡萄酒中人工合成着色剂的快速、准确检测。更多还原

【Abstract】 Aiming to quickly identify whether synthetic colorants are added to wine and accurately quantify, a HPLC method was established to simultaneously detect 11 kinds of synthetic colorants in wine. The experimental samples were diluted with ammonium acetate solution, filtered directly after centrifugation at 8 000 r/min, separated with Zorbax SB-C18column by gradient elution, and detected by multi-wavelength(288 nm, 425 nm, 515 nm,620 nm). The results shown that the method was characterized by simple pre-treatment, no interference from impurities under the maximum absorption wavelength of each colorants, high sensitivity, low detection limit(0.05 mg/L), low limit of quantitation(0.15 mg/L), and good linear relationship(R2≥0.999 4) in the range of 0.20-50 mg/L. The relative standard deviation(RSD) of precision tests results was 1.86%-2.61%, and the recovery rates were 93.0%-103.0%. The method was suitable for rapid and accurate detection of synthetic colorants in wine.更多还原