【摘要】 为提高表面活性素的产量，该研究以具有合成表面活性素能力的枯草芽孢杆菌（Bacillus subtilis）G10菌株为研究对象，利用酸沉淀和甲醇抽提法相结合提取表面活性素，对其表面活性及抑菌性能进行测定，并通过单因素试验和正交试验优化枯草芽孢杆菌G10合成表面活性素的发酵条件及培养基组成。结果表明，表面活性素具有明显的表面活性和抑菌活性，其对大肠杆菌（Escherichia coli）、金黄色葡萄球菌（Staphylococcus aureus）和酿酒酵母（Saccharomyces cerevisiae）的抑菌圈直径分别为1.8 cm、1.9 cm、1.4 cm。合成表面活性素最优发酵条件为：转速200 r/min，发酵温度30℃，发酵培养基初始pH值为7.5；最优发酵培养基组分为：葡萄糖22 g/L,L-谷氨酸5.0 g/L,Mg SO₄0.5 g/L,KCl 0.5 g/L,KH₂PO₄1 g/L,Fe SO₄·7H₂O 0.15 g/L,MnSO₄0.65 g/L,CuSO₄·5H₂O 0.16 g/L。在此优化条件下，枯草芽孢杆菌G10发酵表面活性素平均产量为9.89 g/L，表面活性素产量较优化前提高了46.7%。更多还原

【Abstract】 In order to improve the production of surfactin, Bacillus subtilis G10, which has the ability to synthesize surfactin, was used as the research object, the surfactin was extracted by acid precipitation and methanol extraction, and its surface activity and antibacterial properties were measured.The fermentation conditions of surfactin synthesized by B. subtilis G10 were optimized by single factor tests and orthogonal tests. The results showed that surfactin had obvious surface activity and antibacterial activity, and the inhibition zone diameters of surfactin against Escherichia coli, Staphylococcus aureus and Saccharomyces cerevisiae were 1.8 cm, 1.9 cm and 1.4 cm, respectively. The optimum fermentation conditions for surfactin synthesis were as follows: rotational speed 200 r/min, fermentation temperature 30 ℃, initial pH 7.5, and the optimal fermentation medium components were glucose 22 g/L, L-Glu 5.0 g/L, Mg SO₄0.5 g/L, KCl 0.5 g/L, KH₂PO₄1 g/L, Fe SO₄·7H₂O 0.15 g/L, Mn SO₄0.65 g/L, Cu SO₄·5H₂O 0.16 g/L. Under these optimized conditions, the average yield of surfactin fermented by B. subtilis G10 was 9.89 g/L, which was 46.7% higher than that before optimization.更多还原