【摘要】 目的：探讨S100A10对巨噬细胞介导的炎症及迁移的影响。方法：建立S100A10-KO的C57BL/6J小鼠模型和S100A10-KO的RAW264.7细胞系。取小鼠腹腔巨噬细胞（PMs）和骨髓巨噬细胞（BMDMs），复苏RAW264.7细胞系并收集细胞。采用qRT-PCR检测S100A10敲除对巨噬细胞炎症因子分泌情况的影响；划痕实验和Transwell检测S100A10敲除对巨噬细胞迁移的影响；CCK8试剂盒检测S100A10敲除对RAW264.7细胞增殖的影响；qRT-PCR和Western blot检测基质金属蛋白酶9(MMP9)、非肌性肌球蛋白重链9(MYH9)的表达。结果：S100A10敲除后，RAW264.7、PMs、BMDMs分泌的炎症因子IL-6、IL-1β、MCP-1水平降低（P<0.05）；细胞划痕和Transwell表明S100A10敲除抑制巨噬细胞的迁移；CCK8实验表明S100A10敲除后巨噬细胞的增殖能力减弱；qRT-PCR和Western blot实验表明迁移相关蛋白MMP9、MYH9在S100A10敲除后降低。结论：S100A10敲除后巨噬细胞分泌的炎症因子减少且巨噬细胞迁移和增殖的能力减弱。更多还原

【Abstract】 Objective:To investigate the effect of S100A10 macrophage-mediated inflammation and migration.Methods:C57BL/6J mouse model of S100A10-KO,and RAW264.7 cell line of S100A10-KO were established.Taking mice’s peritoneal macrophages(PMs) and bone marrow macrophages(BMDMs),resuscitating RAW264.7 cell lines,and collecting cells.qRT-PCR was used to detect the effect of S100A10 knockout on the secretion of inflammatory factors in macrophages;wound healing assay and Transwell assay were used to detect the effect of S100A10 knockout on macrophage migration;CCK8 kit was used to detect the effect of S100A10knockout on the proliferation of RAW264.7 cells;qRT-PCR and Western blot were used to detect the expressions of matrix metalloproteinase 9(MMP9) and non-muscle myosin heavy chain 9(MYH9).Results:After S100A10 knockout,the inflammatory factors IL-6,IL-1β and MCP-1 levels(P<0.05) secreted by RAW264.7,PMs and BMDMs were excreted decreased;cell scratches and Trans well showed that S100A10 knockout inhibited macrophage migration;CCK8 experiments showed that the proliferation capacity of macrophages weakened after S100A10 knockout;qRT-PCR and Western blot experiments showed that the migration-related proteins MMP9and MYH9 decreased after S100A10 knockout.Conclusion:S100A10 knockout decreases the secretion of inflammatory factors by macrophages and attenuated the migration and proliferation of macrophages.更多还原