【摘要】 目的：探讨短期香烟烟雾暴露联合poly(I:C)刺激对小鼠肺部免疫应答及干扰素表达的影响。方法：BALB/c小鼠随机分为4组：对照组、熏烟组、poly(I:C)组和熏烟联合poly(I:C)组。检测支气管肺泡灌洗液（BALF）中总细胞数及细胞分类计数；普通光镜下观察各组细胞形态；荧光定量PCR检测肺组织细胞因子、趋化因子和干扰素及干扰素刺激基因表达。结果：与对照组相比，熏烟联合poly(I:C)组总细胞数计数、巨噬细胞与中性粒细胞计数明显升高（P<0.05），且熏烟联合poly(I:C)组巨噬细胞计数高于poly(I:C)组；与poly(I:C)组比较，熏烟联合poly(I:C)组小鼠气道灌洗液巨噬细胞体积较大，呈圆形或不规则形，细胞质较多空泡；与对照组相比，熏烟联合poly(I:C)组小鼠肺组织中性粒细胞趋化因子CXCL1(P<0.05)、CXCL2(P<0.01)和淋巴细胞趋化因子CCL2(P<0.01) mRNA表达升高，肺组织IL-1β、IL-6、TNF-α mRNA表达明显升高（P<0.01），肺组织IFN-β(P<0.01)、IFN-γ(P<0.05)、MX2(P<0.01)和IP-10(P<0.01)表达显著升高，且与poly(I:C)组小鼠相比，熏烟联合poly(I:C)组小鼠肺组织CXCL2(P<0.05)、TNF-α(P<0.01)和IFN-β(P<0.05) mRNA表达明显升高。结论：熏烟联合poly(I:C)诱导了小鼠肺部炎症反应和干扰素及干扰素刺激基因表达。同时，香烟暴露加剧了poly(I:C)诱导的小鼠肺部急性炎症反应和Ⅰ型干扰素表达。更多还原

【Abstract】 Objective:To investigate effects of short-term cigarette smoke exposure combined with poly(I:C) stimulation on lung immune response and interferon expression in mice. Methods:BALB/c mice were randomly divided into 4 groups: control group, smoke group, poly(I:C) group and smoke combined poly(I:C) group. Total cell number and cell classification count of bronchoalveo-lar lavage fluid(BALF) were detected, and cell morphology was observed under ordinary light. Cytokines, chemokines, interferon and interferon stimulating genes expressions in lung tissues were detected by fluorescence quantitative PCR. Results:Compared with control group, total cell count, macrophage count and neutrophil count in smoke combined poly(I:C) group were significantly increased(P<0.05), and macrophage count was higher than that in poly(I:C) group. Macrophages of airway lavage fluid of mice in smoke combined with poly(I:C) group were larger in size, round or irregular in shape, and had more vacuoles in cytoplasm. Com-pared with control group, mRNA expressions of neutrophil chemokine CXCL1(P<0.05), CXCL2(P<0.01) and lymphocyte chemo-kine CCL2(P<0.01) in lung tissues of mice in smoke combined with poly(I:C) group were increased. IL-1β, IL-6, TNF-α mRNA expressions were significantly increased(P<0.01), IFN-β(P<0.01), IFN-γ(P<0.05), MX2(P<0.01) and IP-10(P<0.01) expressions in lung tissues were significantly increased, and compared with poly(I:C) group, mRNA expressions of CXCL2(P<0.05), TNF-α(P<0.01) and IFN-β(P<0.05) in lung tissues of mice in smoke combined with poly(I:C) group were significantly increased. Conclusion:Cigarette smoke combined with poly(I:C) induces lung inflammation and expressions of interferon and interferon stimu-lating genes in mice. Cigarette exposure also increases poly(I:C)-induced acute lung inflammation and type Ⅰ interferon expression in mice.更多还原