【摘要】 目的 建立并使用PCR-序列分析法分析浙江省汉族人群人类白细胞抗原-E(HLA-E)基因的多态性。方法随机选取2024年2至4月浙江省血液中心获捐的150例血小板献血者的外周血样本，抽提标本基因组DNA并采用PCR法扩增HLA-E基因全长序列，利用Sanger测序分析HLA-E基因的第2～4号外显子序列，采用Assign SBT v4.7.1专业软件确定HLA-E基因型。结果 150例浙江省汉族人群外周血标本共检出4种HLA-E基因型，HLA-E*01:01,01:01、HLA-E*01:01,01:03、HLA-E*01:03,01:03、HLA-E*01:03,01:12基因型的频率分别为14.00%、51.33%、32.67%、2.00%；等位基因HLA-E*01:01、HLA-E*01:03、HLAE*01:12的频率分别为39.67%、59.33%、1.00%。结论 PCR-序列分析法以Sanger测序为基础，可实现HLA-E基因分型。浙江省汉族人群HLA-E基因高度保守，多态性较低，仅检出3种HLA-E等位基因。更多还原

【Abstract】 Objective To analyze the polymorphism of human leukocyte antigen-E(HLA-E) gene in Zhejiang Han Population by the PCR sequence-based typing(PCR-SBT) method. Methods Peripheral blood samples of 150 platelet donors in Blood Center of Zhejiang Province were collected during February to April 2024. Genomic DNA of all the samples were extracted and the full-length sequences of HLA-E gene were amplified by the PCR method, and the exons 2-4sequences of HLA-E gene were analyzed by Sanger sequencing. The results of HLA-E genotype were assigned by the Assign SBT v4.7.1 software. Results Four genotypes and three alleles of HLA-E were detected among 150 samples by the PCR-SBT method. The frequencies of genotypes HLA-E*01:01,01:01, HLA-E*01:01,01:03, HLA-E*01:03,01:03 and HLA-E*01:03,01:12 were 14.00%, 51.33%, 32.67% and 2.00%, respectively. The allelic frequencies of HLA-E*01:01, HLA-E*01:03 and HLAE*01:12 were 39.67%, 59.33% and 1.00%, respectively. Conclusion The genotyping of HLA-E can be performed by PCRSBT method based on the Sanger sequencing. The polymorphism of HLA-E gene in Zhejiang Han population is highly conserved and only 3 HLA-E alleles were detected.更多还原