【摘要】 目的 分析2021-2023年广东省疾病预防控制中心收到的食物中毒来源金黄色葡萄球菌(Staphylococcus aureus,SA)耐药特点、分子特征与同源性。方法 使用VITEK 2 Compact全自动微生物生化鉴定仪进行菌株鉴定。使用微量肉汤稀释法进行药敏试验。利用全基因组测序技术揭示菌株的分子流行特征。使用多位点序列分型(Multilocus sequence typing, MLST)与系统发育分析菌株间同源性。结果 8起SA食物中毒事件主要发生在春季(62.5%,5/8);导致中毒的食物类型主要为肉蛋制品(87.5%,7/8)。耐药分析结果表明75株SA均对青霉素耐药，对其他10种药物大部分敏感(76.0%～100.0%);使用耐药基因ErmB、BlaZ、MecA、TetM、Ant6预测SA对红霉素、青霉素、苯唑西林、四环素与庆大霉素耐药性，其总体符合率较高(94.7%)。MLST分析结果显示8起食物中毒来源SA的主要ST型为ST7、ST6与ST45;同一起食物中毒事件来源株同源率为80.5%～100.0%,系统发育进一步提示不同地级市食物中毒来源SA存在传播现象。结论 SA所致食物中毒事件春季高发，肉蛋制品为主要致病食物；食物中毒来源SA均对青霉素耐药，且携带多种毒素基因；同一起食物中毒来源SA同源性较高，不同地区食物中毒来源菌株存在传播现象。更多还原

【Abstract】 Objective This study aims to examine the antibiotic resistance profiles, molecular characteristics, and homology of Staphylococcus aureus(SA) strains associated with food poisoning cases reported to the Guangdong Provincial Center for Disease Control and Prevention from 2021 to 2023. Methods The VITEK 2 Compact fully automated microbiological and biochemical identifier was used for strain identification. Antimicrobial susceptibility was assessed using the broth microdilution method. Whole-genome sequencing provided the molecular epidemiological features of the strains. Multilocus sequence typing(MLST) and phylogenetic analyses were utilized to evaluate strain homology. Results The majority of the eight SA food poisoning incidents occurred during the spring season(62.5%,5/8),with the consumption of meat and egg products as the predominant reason(87.5%,7/8). Antibiotic resistance testing showed that all 75 SA strains were resistant to penicillin, but largely sensitive to the other ten antibiotics tested(Antibiotic susceptibility rate ranged from 76.0% to 100.0%). Predictive concordance for resistance to erythromycin, penicillin, oxacillin, tetracycline, and streptomycin using resistance genes ErmB,BlaZ,MecA,TetM,and Ant6 was notably high, with the predicted compliance rates being 97.3%,90.7%,96.0%,89.3%,and 100.0%,respectively(the combined rate was 94.7%). MLST revealed the primary STs of the food poisoning strains to be ST7(41.3%),ST6(21.3%),and ST45(14.7%). Homology rates among strains from the different food poisoning cases ranged from 80.5% to 100.0%. Phylogenetic analysis indicated the presence of transmission phenomena among SA strains from different prefecture-level cities. Conclusion Incidents of SA-induced food poisoning were found to peak in the spring, with consumption of meat and egg products being the principal modes of transmission. All SA strains from the food poisoning cases demonstrated penicillin resistance and harbored multiple toxin genes. Strains from the same incident exhibited a high degree of homology, while transmission was observed between strains from various regions.更多还原