【摘要】 双力育种技术是利用叶片的电学行为，获取作物的胞内水代谢、物质代谢和能量代谢等植物生理动力学参数，表征作物生命力和生产力(双力)。以“双力”为评价指标在线快速评价作物的生长机能、发育机能、代谢能力和抗逆机能等，对常规育种中的表型和株系、杂交育种的后代以及分子育种的基因型实现在线快速筛选和评价。利用双力育种技术，对16份仿野生附树栽培铁皮石斛资源的叶片胞内水代谢、营养转运能力以及代谢活力进行综合评价，依据得分高低从中筛选出5份适应喀斯特林地附生干燥树皮的铁皮石斛优异株系。继续对得分最高的3份铁皮石斛资源进行筛选和评价，最终获得两次排名均为第一的适宜仿野生干旱逆境铁皮石斛品系LH1,表明双力育种技术能使优良株系筛选效率得到极大的提升。更多还原

【Abstract】 Dual-capability breeding technology uses the electrical behavior of leaves to obtain plant physiological and dynamic parameters such as intracellular water metabolism, material metabolism and energy metabolism of crops to characterize crop vitality and productivity(Dual-capability). The growth function, development function, metabolic ability and stress resistance of crops were rapidly evaluated online with ’Dual-capability’ as the evaluation index, and the phenotype and strain in conventional breeding, the offspring of cross breeding and the genotype of molecular breeding were quickly screened and evaluated online. The intracellular water metabolism, nutrient transport capacity and metabolic activity of 16 Dendrobium officinale resources were evaluated by dual-capability breeding technique, and 5 excellent Dendrobium officinale lines adapted to the epiphytic dry bark of karst forest were selected according to their scores. The three Dendrobium officinale resources with the highest scores were screened and evaluated, and finally LH1, which ranked first for both times, was suitable for simulating wild drought stress, indicating that dual-capability breeding technology could greatly improve the screening efficiency of excellent strains.更多还原