【摘要】 Dirigent(DIR)蛋白在木质素的合成中发挥重要作用，同时还能提高植物对各种病原体的抗性。本研究以克隆的杜仲Dirigent1基因(EuDIR1)为基础，构建EuDIR1过表达载体遗传转化番茄，获得转基因植株后分析EuDIR1基因在番茄抗灰霉病中的作用。结果表明，与野生型和转空载番茄相比，接种灰霉菌后过表达EuDIR1基因的番茄发病更晚，病斑直径显著小于野生型与转空载植株，说明EuDIR1显著提高了番茄植株对灰霉病的抗性。过表达EuDIR1基因还可提高番茄植株接菌前后的保护酶活性与番茄病程相关蛋白(PRs)的基因表达，从而增强植株对灰霉病的抗性。此外，EuDIR1基因过表达后番茄叶片和茎的木质素含量都显著增加，且木质部导管附近的细胞壁显著增厚，推测EuDIR1基因可能通过增加番茄中木质素的含量，增厚番茄木质部导管附近的细胞壁来增强番茄对灰霉病的抗性。本研究揭示EuDIR1基因在番茄抗灰霉病中的功能，可为番茄抗病育种提供理论依据和利用途径。更多还原

【Abstract】 Dirigent(DIR) proteins play an important role in the synthesis of lignin, which improve plant resistance to various pathogens. In this study, based on the Dirigent1 gene of Eucommia ulmoides(EuDIR1) cloned in the laboratory, the EuDIR1 overexpression vector was constructed and transformed into tomato. After obtaining the transgenic plants, the role of EuDIR1 gene in tomato resistance to Botrytis cinerea was analyzed. The results showed that compared with wild type and unloaded tomato, tomato with overexpression of EuDIR1 gene after inoculation with Botrytis cinerea occurred later, and the lesion diameter was significantly smaller than that of wild type and and plants transferred into the empty vector, indicating that EuDIR1 significantly improved the resistance of tomato plants to Botrytis cinerea. Overexpression of EuDIR1 gene could also increase the protective enzyme activity and the expression of tomato course-related protein(PRs) gene before and after inoculation, thus enhancing the resistance of tomato plants to Botrytis cinerea. In addition, after overexpression of EuDIR1 gene, the lignin content in tomato leaves and stems increased significantly, and the cell wall near the xylem vessel significantly thickened. It was speculated that EuDIR1 gene could enhance tomato resistance to Botrytis cinerea by increasing lignin content in tomato and thickening the cell wall near xylem vessel. This study revealed the function of EuDIR1 gene in tomato resistance to Botrytis cinerea, which could provide some theoretical basis for tomato disease resistance breeding.更多还原