【摘要】 目的 观察经皮穴位电刺激(transcutaneous electrical acupoint stimulation, TEAS)对去势后SD大鼠血清骨代谢指标及骨密度(bone mineral density, BMD)的影响。方法 大鼠随机分为Sham组(假手术组)、0VX组(模型对照组)、2 Hz组(0VX+TEAS 2 Hz组)、50 Hz组(0VX+TEAS 50 Hz组),每组8只，3个月后分别检测血清骨代谢指标骨特异性碱性磷酸酶(BALP)、I型原胶原C端前肽(PICP)、I型原胶原N端前肽(PINP)、I型胶原羧基端肽(ICTP)、吡啶啉(Pyr)、脱氧吡啶啉(D-Pyr)及BMD。结果 0VX组较Sham组血清BALP、PICP、PINP、ICTP、Pyr、D-Pyr均显著增高(P<0.05);与OVX组比较，2 Hz组血清BALP、PINP、PICP、ICTP、Pyr、D-Pyr均显著降低，腰椎、右股骨BMD增加(P<0.05),50 Hz组血清BALP、PINP、PICP及BMD无明显变化，ICTP、Pyr、D-Pyr亦均显著降低(P<0.05);与50 Hz组比较，2 Hz组腰椎BMD增高(P<0.05)。结论 经皮穴位电刺激可能通过调控骨吸收和骨形成指标，参与抗骨质疏松作用，且2 Hz电刺激频率可能较50 Hz更适用于临床骨质疏松症的防治。更多还原

【Abstract】 Objective To observe the effects of transcutaneous electrical acupoint stimulation(TEAS) on serum bone metabolic indexes and bone mineral density(BMD) in ovariectomized SD rats. Methods Thirty-two rats were randomly divided into Sham group(sham operation group), OVX group(model control group), 2 Hz group(OVX+TEAS 2 Hz group), and 50 Hz group(OVX+TEAS 50 Hz group), with 8 rats in each group. Serum bone metabolic indexes, including bone specific alkaline phosphatase(BALP), type I procollagen C-terminal propeptide(PICP), type I procollagen N-terminal propeptide(PINP), type I collagen carboxyl-terminal peptide(ICTP), pyridinoline(Pyr), deoxypyridinoline(D-Pyr), and BMD were detected 3 months later. Results Compared to those in Sham group, serum BALP, PINP, PICP, ICTP, Pyr, and D-Pyr in OVX group increased significantly(P<0.05). Compared to those in OVX group, serum BALP, PINP, PICP, ICTP, Pyr, and D-Pyr in 2 Hz group decreased significantly. BMD in lumbar spine and right femur increased(P<0.05). BALP, PINP, PICP, and BMD in 50 Hz group had no significant changes. ICTP, Pyr, and D-Pyr also decreased significantly(P<0.05). Compared to that in the 50 Hz group, the lumbar BMD in the 2 Hz group was higher(P<0.05). Conclusion TEAS play a role in anti-osteoporosis, probably by regulating bone resorption and bone formation indexes. The electrical stimulation with frequency of 2 Hz may be more suitable for clinical prevention and treatment of osteoporosis than with 50 Hz.更多还原