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低温/中温花生粕蛋白的理化特性及结构特性分析

Analysis of Physicochemical and Structural Chracteristics of Low-Temperature/Medium-Temperature Peanut Meal Protein

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【作者】 张彤冯雨禾戚朝霞郭浩曾里贾利蓉段飞霞冉旭

【Author】 ZHANG Tong;FENG Yu-he;QI Zhao-xia;GUO Hao;ZENG Li;JIA Li-rong;DUAN Fei-xia;RAN Xu;College of Biomass Science and Engineering, Sichuan University;Key Laboratory of Food Science and Technology in Institutions of Higher Education in Sichuan Province, Sichuan University;

【通讯作者】 曾里;冉旭;

【机构】 四川大学轻工科学与工程学院四川大学食品科学与技术四川省高校重点实验室

【摘要】 对低温/中温花生粕的感官色差、功能特性和结构特性进行了研究和分析,旨在为提高花生粕的综合利用价值提供理论基础。结果表明,低温花生粕呈象牙色,中温花生粕呈灰白色。从功能特性上来看,低温/中温花生粕蛋白的持油性、乳化性和泡沫稳定性无显著性差异。低温花生粕蛋白具有更好的溶解度、起泡性、持水性、热稳定性,中温花生粕蛋白具有更好的乳化稳定性。进一步通过分子量、二级结构、DSC对蛋白质结构进行分析,低温花生粕和中温花生粕蛋白质二级结构主要是β-折叠、β-转角,中温花生粕蛋白变性程度更高,低温花生粕的蛋白结构相对更松散。低温花生粕蛋白具有更高的变性温度和热焓值,且具有更好的热稳定性。

【Abstract】 The sensory color difference, functional characteristics and structural characteristics of low-temperature/medium-temperature peanut meal are studied and analyzed, in order to provide a theoretical basis for improving the comprehensive utilization value of peanut meal.The results show that low-temperature peanut meal is ivory tint, and medium-temperature peanut meal is grayish white. In terms of functional characteristics, there is no significant difference in the oil-holding capacity, emulsibility and foam stability of low-temperature/medium-temperature peanut meal protein. Low-temperature peanut meal protein has better solubility, foamability, water-holding capacity and thermal stability, while medium-temperature peanut meal protein has better emulsification stability. The protein structure is further analyzed through molecular weight, secondary structure and DSC. The secondary structure of low-temperature peanut meal protein and medium-temperature peanut meal protein is mainly β-folding and β-turning. The denaturation degree of medium-temperature peanut meal protein is higher, and the structure of low-temperature peanut meal protein is relatively looser. Low-temperature peanut meal protein has higher denaturation temperature and enthalpy, and better thermal stability.

【基金】 四川大学青岛研究院“8122计划”项目(21GZ30201)
  • 【文献出处】 中国调味品 ,China Condiment , 编辑部邮箱 ,2024年04期
  • 【分类号】TS201.21
  • 【下载频次】166
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